DOCUMENT SOURCE Lawrence Berkeley Laboratory Archives and Records Office RECORDS SERIES TITLE Applied Science Division: Scientists’ Papers - Papers of William E. Siri ACCESSION NO. 434-91-0131 FILE CODE NO. 19-14-18 CAATON NO. 7 FOLDER NAME _ 44 OE COPY Dr. Leo Meyer (Marshall Island Natives} NOTES ie FOUND 8Y cegese Peny Hall Ai eeeeeemmweeee 8 8 poe mere is the procedure on tho use of tritiated water for measuring total body water. 1. Woe will provide the NTO. etreebypetecei oe 3 2 ae ah ae S a 2 “(Has led oe ko Give by mouth 1 ce of HTC. 7 a ate, cick Feb, [ 66 4 Pere Poe peers He cere tt ee “Vransfer from stock bottle to a “mtlass, cup, or beaker ane, exactiy 1,00 ce HTO with a tuberculin syringe (or 1 ee pipette), C te BEST COPY AVAILABLE =a Fis) Pett Add 50-100 ec water and have sub-_ cet drink entire contents. ‘dé another 50-100 cc water to the vessel and again have subject drink entire contents. 2. Tritium dilution can be ceturmined in either plasma or urine ‘Cor both). if urine samples are collcecicd, follow this procedure: (a) “Nave subject void about & = after taking tritium. (b) Take iC ce urine samples at aporoximately 5 nrs., 8 hrs., and 12 prs. after taking tritium. can be obtained. «2: hr, sample is useful if tt Put the urine sample in a 3 or 5 dram vial Wil a erystal of thymol and labcled with name or code nunber, date and time after administraticn of ITO. if a plasma sample is urine), eo beuwe taxen (in nke the sarnple 4+ temwemsmme. after ving tne tritiua. rut £ ec of plasma (or sorus, Ins vinl with a erystal of thymel, and an identifying label. -£ convenient, we store sonples cold or frozen, yut ust ossewtiad w= sure to get weleht and helsht of subjects on day total \, — So Return the urine (and/or >ltsna) samples assayve you « ew 1 an 7 s oa s . s “@ WL1L determine the total body water values and mail the results. eso ~ne cc of our stock HTO scntains about 25® pre tritium. assuming & normal blological hallt-time for turnover, the accunudative radiation cose in a 75 Urn. man is about #millirads. wa? ,