DOCUMENT SOURCE
Lawrence Berkeley Laboratory
Archives and Records Office
RECORDS SERIES TITLE
Applied Science Division: Scientists’ Papers - Papers of William E. Siri
ACCESSION NO.
434-91-0131
FILE CODE NO.
19-14-18
CAATON NO.
7
FOLDER NAME
_
44 OE
COPY
Dr. Leo Meyer (Marshall Island Natives}
NOTES
ie
FOUND 8Y
cegese
Peny Hall
Ai eeeeeemmweeee
8 8 poe
mere is the procedure on tho use of tritiated water for
measuring total body water.
1.
Woe will provide the NTO.
etreebypetecei
oe
3 2
ae
ah
ae S
a
2
“(Has led
oe
ko
Give by mouth 1
ce of HTC.
7
a
ate,
cick
Feb, [ 66
4
Pere Poe
peers
He cere tt
ee
“Vransfer from stock bottle to
a “mtlass, cup, or beaker ane,
exactiy 1,00 ce HTO with a tuberculin
syringe (or 1 ee pipette),
C te
BEST COPY AVAILABLE
=a
Fis)
Pett
Add 50-100 ec water and have sub-_
cet drink entire contents.
‘dé another 50-100 cc water to the
vessel and again have subject drink entire contents.
2.
Tritium dilution can
be ceturmined in either plasma or urine
‘Cor both).
if urine samples are collcecicd, follow this procedure:
(a)
“Nave subject void about & = after taking tritium.
(b)
Take iC ce urine samples at aporoximately 5 nrs., 8 hrs., and
12 prs. after taking tritium.
can be obtained.
«2: hr, sample is useful if tt
Put the urine sample in a 3 or 5 dram vial
Wil a erystal of thymol and labcled with name or code nunber,
date and time after administraticn of ITO.
if a plasma sample is
urine),
eo beuwe
taxen
(in
nke the sarnple 4+ temwemsmme. after
ving tne tritiua.
rut £ ec of plasma (or sorus, Ins vinl with a erystal of thymel,
and an identifying label.
-£ convenient,
we
store sonples cold or frozen, yut ust ossewtiad
w= sure to get weleht and helsht of subjects on day total
\,
—
So
Return the urine (and/or >ltsna) samples
assayve
you
«
ew
1
an
7
s
oa
s
.
s
“@ WL1L determine the total body water values and mail
the results.
eso
~ne cc of our stock HTO scntains about 25® pre tritium.
assuming & normal blological hallt-time for turnover, the accunudative radiation cose in a 75 Urn.
man is about #millirads.
wa?
,