7-7

a

-™

population at an essentially linear rate of 0.4 ~ 0.5% per generation, a
rate consistent with known mutation rates. In the populations treated with

a large dose of x-rays (7000r for males, 1000r for females), the lethal
chromosomes dropped from an origingl fequency of 18% to 10%.4n four

generations and subsequently have increased in frequensy at a rate comparable

to that of the control,

The initial decrease is that expected from the

elimination of translocations which were coincident with recessive lethals.

It seems, therefore, that induced recessive lethals which are not associated

with translosations have remained in the pepulation and are included in the
concealed variability of the population, whereas those induced lethals which
were associated with translocations were eliminated rapidly as a result of

the concomitant reduction in fertility associated with thetranslocations,

To determine the frequency of allelisa of recessivelethals, a primary

source of elimination of these lethals from any population, series of cross

tests were made between lethals (induced by gawea s-rays) af independent
origin, The mmber of lec! calculated from our results is 400 with possible

values ranging fran 23% te 718 (99% confidence interval). This number does

not differ significantiy from that determined by Ives from atudying lethals
of spontaneous origin. The magnitude of this number makes it highly

improbable that any appreciable elimination of recessive isthals has ceccurred
through homozygosity.
Analyses of the viabilities of those files hososygous for non-lethal,

non-eemi-lethal chromosomes (i.e., the bulk of the tested chromosomes which
seex to be "normal" at first glance) reveal that their average viability is

relatively constant but
varianee of their distribution,

analyses indicate an increase in the
This implies that a coumteraction of mutation

of deleterious genes (sub~vitals) and the selection for advantageous genes

(gene combinations) have, to date maintained the constant average viability.
Since, however, the detailed analyses of this problem have just begun, it is

best that this conclusion be taken with some reserve. It seems highly

probable that this mechanism is operative in the contrel population but it
ia too early to discuss the experimental populations.

Gamma Radiation.

A conference was held at Oak Ridge June 6 to consider a project

originating with the Toxicity Laboratory of the University of Chicago on the
effects of gamma radiation on mamzals. The project sponsored jointly by the

Division ef Biology and Medicine and the Division of Military Application was

originally planned to bs carried out at Dugway. Since burros are adaptable
to the enviroment at Dugway and are approxinately the size of man, they will
be used as the experimental animal, The animals will beirrediated with
tantalum. Jtudles will be made on the effect of radiation on sperm physlotogy,
work capacity and blood sonstituents.

CoORnte

\
ey

ero

16--56741-1

OFFICE > |
&.”

MEP
=>

| DOR ARCHIVES —

Select target paragraph3