38 phosphatase staint#Potneutrophils showed a generally normal disgeibution of positive cells, as in the previous year. Basophil counts of #000 cells showed noincrease in levels above the norma! percentage in any subjects, either exposed or unex- posed, in the 1959 and 1960 survevs, except for one 6-year-old exposed boy ( =3).* The mean percent basophil count (counting 4000 white cells} for tHe exposed Rongelap group was 0.42% in 1959 and 0.93% in 1960; in the Ailingnae group, 0.29% in 1959 and 0.80% in 1960. Basophil counts on the unexposed group are not yet complete for 1959. The individual values for alkaline phosphatase and basophil counts are presented in Appendix 5. Complement Fixation Tests The results of the complement fixation tests are shown in Table 23. In discussing these results Dr. R. J. Heubner made the following comments: “IT suppose the most interesting thing is that there ts evidence of infection with almost all the groupsof viruses that wetestedfor. It is important to point out that complement-fixing antibodies are generally less persistent than are hemaggiutination inhibition and neutralizing antibodies. Therefore, the high levels of adenovirus and HA 1 complement-fixing antibodies reflect either recent infection or multiple infections with various members of the parainfluenza family of viruses. The low levels obtained for other agents, such as influenza A, influenza B, HA 2, CA, mumps, LGV, and © fever may reflect low levels of infection with these agents, but morelikely might reflect the fact that such agents have not been prevalent in recent years. Thus, one might conclude from the data that the Asian influenza virus has not yet seriously involved the Marshall Island populations,or else, of course, that the complement-fixing antibody produced bythe Asian influenza has notpersisted. ‘‘[ would rather suspect from the age distribution that Asian influenza still has not reached these people, and that one might look forward to see if they are tnvoived at some future date. Sim- ilarly, the age distribution of influenza B antibodies suggests that it has not occurredfor at least 6 to 10 years. The absence of HA 2 antibodies suggests that the virus has not been prevalent in re- “In 1959 his basophil count was 2.1%, WBC 8600, and alka- line phosphatase negative cells 79%. In 1960 his basophil count was 3%, WBC 6100, and alkaline phosphatase negative cells 70%. No abnormal forms were seen. 1185588 cent vears. However, the total absence of anti- bodies even in persons over +0 suggests the possibility that it has not occurred in this population at all. but the frst interpretation ts equally good. The same comments would refer to the CA virus or parainfluenza 2, but here the morelikelyinterpretation ts that the virus has not been present in the recent past, but the presence of antibodies may suggest that it had been present 16 or more years earlier. Since there is some overlap in antigens and antibodies between mumpsand the parainfuenza group, the CA reactions and/or the mumpsreac- tions could conceivably merelyreflect infections with other members of the parainfluenza group. ‘It would be interesting to find out to what extent the psittacosis group and fever represent a threat to this population, and of course in the birds and domestic animals as well.” Dr. Heubner’s forecast of Asian influenza in the Marshall Islands from the Rongelap data is inter- esting in that an epidemic of serious proportions did occur at Kwajalein Atoll in January 1960 resulting in significant mortality. ° The average antibodytiters for the different age groupsin the exposed people were, in nearly all cases, lower than in the corresponding unexposed age groups. It is not known whetherthis is a significant finding; further studies will be done to determinethis. At three years after exposure, the primary response to tetanus antitoxin averaged slightly less in the exposed people than in the unexposed.*° However, because of the smail numbers of people studied, this finding could not be considered significant. Sodium and Potassium Levels, 1959 Potassium intake appeared to approximatethatof Americans, namely, 40 to 100 mEq/day. Salt intake varied widely as judged by urine excretion, ranging from 2 to 18 g/day. The relatively high salt consumption of some individuals is undoubt- edly related to the irregular consumption of canned C rations, to which 2% NaCl is added routinely during preparation. The median intake of salt appeared to approximate that of male employees at Brookhaven National Laboratory, namely, 210 g/day. This undoubtedly represents an increase over the intake in earlier years when only native foods were eaten. It will be of interest to note whether the tncidence of hypertension also will begin to increase. At present the prevalence of hypertension (B.P. greater than 140/90) is 9.2%

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