leve1.° - trophic Tuna Euthynnus and mackerel nuda Gymnosarda and Jacks crustacea. benthonic on primarily Grammatorcynus the. fourth in are snappers Thunnus albacares, billineatus are (bonito), affinis feeding is a bottom dweller Another snapper Letherinus kallopterus (pigfish) and large, rapid-swimming carnivores feeding on small fish and any other prey of proper They represent species of the fifth trophic leve1.® size. SAMPLE PROCESSING AND ANALYSIS The fish samples partially thawed. fish was from location The total weight, determined. Each (cranial and thoracic, skin and each scales fish numerically standard or fork length, was dissected vertebrae and ribs, (fins were discarded), into counted and and sex of each muscle tissue, bone and pelvic and pectoral girdle), stomach contents, liver, and remaining viscera that included large and small intestine with contents, stomach wall, spleen, organ kidney, and mesenteries. Each separate species from the same catch was pooled. but not analyzed. Our experience be little possible academic dissected. viscera Adductor that Clams were muscles, included from 1978 showed plus gonad, measured siphon, stomach, because (total kidney, intestine from smaller clams such as the Tridacna crocea from specific for analysis. individuals. For the larger species, After the wet weight was determined, sample was dried in ovens at fish gills were of the length), and and remaining and contents, crystalline style, heart, and nervous system were removed for analysis. pooled the from the the their analysis weighed, mantle gills, to of The gills are not eaten and there could information gained contamination. and Gills were separated prior sometimes contaminated with sediment. tissue analysis Parts locations were was made on each fish and clam tissue 90°C to constant dry weight and dry ashed in muffle furnaces at 450°C for approximately 72 h. Samples were transferred to aluminum containers, gamma spectrometry. Gamma-spectrometry sealed, and analyzed by measurements were made on all separated samples at LLNL using a variety of Ge (Li)-diode detector systems. Counting times were usually 1000 min or longer for each sample. A general-purpose reduction long-lived of all nuclear computer generated explosion program, spectra. products, GAMANAL, The program activation was used searches products, for a and the library data of naturally