10. Prepare a cation exchange column with a 12mm X 18em bed of BioRad 50WX8, 50-100 mesh resin. Wash the column with 20 ml 8M HNO3 followed by 25 mi of deionized water. Rinse column with 25 ml of 0.5M HNOg3. 11. Pass sample through resin column. Wash column with 25 ml of 0.5M HNOs, then with 100 ml of 1.5M HNO3followed by 20 ml of 2M HNO3. 12. Elute the americium into a 250 ml beaker with 80 m1 of 6M HNOg followed by 20 mi of 8M HNO3. Evaporate the sample to near dryness. 13. Dissolve the sample in 5 ml of 8M HNOg andtransfer into a 40 m1 centrifuge tube. Rinse the beaker with two 5 ml portions of deionized water and add rinse to centrifuge tube. Add approximately 10 mg of Fe carrier. 14. Adjust pH to 9-11 with 12M NaOH and digest in a hot water bath for 5 minutes. Cool sample, centrifuge and discard supernatant (Note 1). 15. Dissolve the precipitate in 5 ml cone HNOg and a few drops of cone HCl. Digest in a hot water bath for 5 minutes. Add 20 ml of deionized water and repeat Steps 14 and 15. 16. Adjust pH to 9-11 with cone NHgOH and digest in a hot water bath for 5 minutes. Cool sample, centrifuge and discard supernatant. Dissolve the sample in 15 ml of eone HCl and 1 drop cone HNOg3. 17. Repeat Steps 7 and 8. 18. Add 5 ml of cone HNO3 and evaporate to near dryness. DO NOT BAKE. 19. Electrodeposit sample as follows: a. Add 2 ml of 0.4 N HCl to the beaker. b. Add 3 ml of 4% ammonium oxalate solution. Swirl. ec. Transfer the electrolyte sample mixture into a numbered plating cell with deionized water. Add rinses to cell until cell is full (1/8" from top). d. Electrodeposit at 210 ma for 2.5 hours. 20. After 2.5 hours of electrodeposition and with current still on, add two drops of phenolphthalein indicator and make basic with 1% NH4OH until pink color appears. 21. Removethe plating cell and wash with two 10 ml washes of deionized water. Removethe disc from the plating cell, rinse once with alcohol and flame over a Bunsen burner. 22. Aliow dise to cool and count 400 minutes on the alpha spectrometer. 23. Calculate dpm of 241Am per sample as follows: a. Add the net counts within the 2414m channels to obtain total net counts. A-11-3