10 Dental Survey. Intra-oral examinations were conducted as part of the physical examination.All patients were examined with mouth mirror and explorer. Ilumination was by standard operating light. Salivary pH was taken by use ofpHydrion on all subjects who were not eating candyor any other foodstuff. Saliva samples were collected from these patients at the time pH determinations were made. Intra-oral radiograms were not available. Dental examinations were carried out on thefollowing groups: 30 children at Majuro; all the adults.and children at Rongelap whowerein the physical examination group; and a numberof children at Utirik. Studies of Aging Criteria Although the exposed group has shown no outward evidence visible by gross observation of any accelerated aging effect of radiation, it was thought desirable to have available measurable criteria of possible age changesfor use during normal physical examination. Therefore the over-all objective of this study was to establish tests to evaluate any possible radiation-induced senescence in the exposed group compared with the unexposed population living on the same island. However, the data (presented in a later section on results) collected during the 1959 survey (5 years after exposure) showed no apparent differences between the exposed and unexposed groups. Therefore the data from the two groups have been pooled with the objectives of presenting the methodology, indicating the trend of changes with age of the various aging criteria chosen, and attempting to determine a biological age score for individuals anddifferent age groups. This study was hampered bythe smal! number of people involved and the lack of vital statistics on the Marshallese people. Another difficulty has been the uncertainty of exact ages in some cases, particularlyin older people. Data on aging criteria were recorded only on adults 20 years of age and over. Of the 126 adults, 42 were in the originally exposed group and 84 in the larger comparison population. Table 1 shows the age and sex distribution. The ages were reason- ablywell distributed except for a smaller number of older people (> 60 years of age). The age criteria chosen were based on changes generally believed to be associated with physiological senescence and represent onlya small number of the possible ones. Theywere selected witha Table 1 Age Distribution in Rongelap Adult Population, 1959, Used in Aging Study Exposed Unexposed Age, yr M F M F 20-24 25-29 30-34 35-39 40-44 45-49 90-54 55-59 60-64 65-69 70-74 75-79 >80 0 2 3 1 4 1 1 2 0 0 1 0 2 5 1 2 4 2 0 0 I 6 0 2 1 1 1 12 3 4 6 2 5 3 5 2 0 0 2 6 6 5 4 3 3 5 1 3 3 0 0 0 Total 12 21 13 13 15 6 MH 7 14 3 3 1 5 126 DOE ARCHIVES” view toward ease of assessment during routine physical examination underfield conditions, time limitations, and Janguagebarrier. Therefore, unfortunately, tests of vigor and functional capacity were necessarily limited. * Of the 15 criteria selected, 9 were measured directly and 6 wereestimated on a 0 through 4+ scale. Five tests involved the integument: (1) skin looseness, (2) skin elasticity (retraction time), (3) senile changesin the skin, (4) greying ofthe hair, and (5) baldness. Four tests involved the special sense organs: {1) accommodation, (2) visual acuity, (3) arcus senilis, and (4) hearing. The cardiovascular system was tested by(1) systolic and (2) diastolic blood pressure recordings, (3) peripheral arteriosclerosis, and (4) retinal arteriosclerosis. There was one test of neuromuscular function. Vigor was measured by hand strength measurements. The test methods are described below. Infegument. A special skin caliper was de- signed for measuring skin looseness andelasticity (Figure 8). The legs of the caliper can be opened to anydesired degree up to 5 cm on scale A byadjusting screw B. The spring tension whenthecaliper is closed on a fold of skin results in a pressure of about 500 g. The inner and outer surfaces of the . Jegs are calibrated in millimeter markings (C). *Several tests, such as pulse and blood pressure response to a two-step test and vital capacity, were tied but not used because they proved unsatisfactory.