419 oo ‘ to that of the phantom, which was counted for 30 min (Figure 52). In future whole-body counting of these people, it will be possible, by counting for longer periods and using an 8-in. Nal crystal, to improve the absolute measurement of trace amounts of other radionuclides that may be present. Since a total of 227 Marshallese persons were surveyed with the whole-body counter, in addition to numerouscontrols, the spectral analyses were performedwith the aid of a 704 IBM computer. Radiochemical Procedures Twenty-four-hour urine specimenswere collected in plastic bottles and sent to BNLfor radiochemical analysis. A modification of the method of Farabee*® was used for the analysis of Sr®°. Sr was precipitated as the alkaline phosphate, ashed with HNO, and H,O,, and dissolved in dilute HNO,,. After the solution was brought up toa {- RESULTS AND DISCUSSION ' All three of the above methods were used for estimating the body burdens of gamma- andbetaemitting radionuclides in the Marshallese people. Individual values for all the people examined in 1959 may be found in Appendix 7 for gamma spectrographic analyses and in Appendix 8 for radiochemical analyses. Environmental Estimate DOE ARCHIVES One methodused (the least quantitative) was the environmental estimate of body burden. The environmental estimate of internally-deposited Sr°° was made in two ways. In the first method, animals subsisting on diets similar to human diets weresacrificed and their tissues were analyzed radiochemically. A numberofrats were collected on RongelapIsland at 2, 4, and 5 years after the 1954 accident. If the diet of these rats, primarily plexed with EDTA,and the pH was adjusted to 5.5. The solution was then passed through an ion land plants, was sufficiently similar to the diet of humanbeings inhabiting this area, the rat analyses mightserve as indicators of the humaninternal tion of 1% citric acid and 0.75% EDTAat a pH of various tissues of these rats were measureddirectly and comparedto the ratios of the food andsoil volume of 800 cc, the alkaline earths were com- exchange column (Dowex-50 in the Na form), and the column was rinsed with 300 cc of a solu- 5.0. The combined effluents contained >95% of the total Ca. The column wasthen rinsed with 6 N HNO,to removethe Sr*°. Carrier Sr was added to the Sr® fraction and precipitated with 70% fuming HNO,,. Yttrium-90 was milked and counted by the method of the AEC Health and Safety Laboratory.** The supernatantfrom the alkaline phosphate precipitation was measured and divided into two portions. One portion was scavanged for cesium with added carrier by means of a double precipitation of the aluminum sulfate and the chloro- platinate.”? The second portion was analyzed for K by flame spectrophotometry. Food samples were weighed and dry-ashed ina muffle furnace at 800°C. The ash was weighed, and a small portion was counted for grass beta activity. The ash was dissolved in dilute HNO, and processed by the method described abovefor urine analysis. All counting was done in a low-level beta anti- coincidence type of counter, designed and built at BNL. Samples were mounted on glassfiber filter discs with nylon rings and discs and Mylarfilm. Samples were counted against NBS standards processed and counted underidentical geometry.*" Tr rapemw ee eee radiation contamination. The Sr°°/Caratios of on Rongelapcollected at the same time; thatis, the environmental contamination was compared with the directly measured contamination in animal tissue. Extrapolation of the environmental data gives the equilibrium value which can be expected, whereas the direct measurement gives the value at the time of measurement (and thus the percent of the equilibrium value for the individual radionuclides). The Sr°°/Caratios for different plant foods on Rongelap varied greatly, and the diet of the rats was too uncertain for an “average” diet to be assumed. Therefore, for a body burden estimateit was necessaryto use the Sr°°/Ca values ofthe soil itself. The “‘strontium-calcium observed ratio” (OR) of Comar*? was used to denote the preferential utilization of calcium in the following manner: OR gampie-precursor = Sr/Ca of sample Sr/Ca of precursor The Sr®° discrimination ratio in the chain from soil (s) to bone (6) via plants ( ) can be expressed as follows: OR,_,=(OR,_,)(OR,.,)=(0.7)(0.25)=0.18. ee Oe ee ee eee

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