diluted
Erythrocyte Count--Blood was drawn into an RBC pipette (+1%) and

with Haymen's dilution fluid (1:200). A Specto-Bright-line hemocytometer
was used for counting erythrocytes. Numbers were estimated in millions/cm?.
Leukocyte Counts--Blood was drawn into a WBC diluting pipette (+1%) and

diluted (1:20) with 1% glacial acetic acid.
cytometer was used for counting leukocytes.
thousands/cm?.

A Specto-Bright-line hemoNumbers were estimated in

Plasma Protein--Plasma from the packed cell volume test was utilized to
estimate concentration of plasma proteins using an American Optical total
Total plasma proteins were read
solids meter No. 10400 Refractometer.

directly on the meter (g/100 ml).

Albumin concentration was determined colormetrically using an American
Monitor buffered albumin dye No. 1007. The plasma albumin binds quanti-

The
tatively to the day 3, 3', 5, 5'-tetrabromo-m-cresolsulfonphthalein.
albumin dye combination produces an intense blue chromophore which was
A standard curve
measured at 600nm on a Bausch and Lomb Spectronic 20.
was developed for each sample series, and the albumin concentration

calculated in gm/100 ml.

Glucose--Glucose concentration was determined using Harleco reagent and
standard set No. 64147.
To 10.0 ml reagent (ortho-toluidine 6% in glacial

acetic acid), 0.1 ml of plasma was added. After boiling, the resultant
green color was measured photometrically on a Bausch and Lomb Spectronic
20 at 630 nm, and the concentration (mg/100 ml + 2 mg/100 ml) was determined
from a standard curve.
Plasma Cholesterol--Plasma cholesterol was determined colormetrically

using Harleco standard 7653B and reagent 7653A (acetic acid, acetic
anhydride, and sulfuric acid). Plasma (0.1 ml) was added to 5.0 ml of
reagent and incubated for 10 minutes at 37° C.

The resultant color is

proportional to the cholesterol concentration and read on a Bausch and
Lomb Spectronic 20 at 625 nm.

RESULTS AND DISCUSSION

Concentrations of 239Pu and 24%1am in Small Vertebrates of NAEG Intensive
Study Areas

Small vertebrates found in six NAEG Intensive Study Areas of NTS and TTR have
been previously reported (Moor and Bradley, 1974; Bradley and Moor, 1975) and,
in general, species composition agrees with earlier investigations of vertebrates from comparable habitats of NTS outside of the Study Areas (Hayward et

al., 1963; Tanner and Jorgensen, 1963; Jorgensen and Hayward, 1965).

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