other purposes.

Aboveground activity of each species in each area was based

on the relative number of individuals captured seasonally; reproductive status
and recruitment were estimated by the percentage of the populations which were
described as either sexually active adults, sexually inactive adults, young
adults, or immatures.
Radioanalysis

Individual animals known to be residents in the Study Areas for at least three

months were captured, sacrificed, and taken to the CETO building in Mercury,
where they were autopsied. Animals were also collected off-site for analysis
following the same procedures used in NAEG Study Areas. Tissue aliquots for
radioassay included pelt or skin, GI tract, and carcass. Laboratory procedures
for preparation of tissue samples have been reported (Moor and Bradley, 1974;
Moor et al., 1976b) and included dipping animals in hot paraffin wax to minimize

Levels of 239py and 241Am
cross-contamination between respective aliquots.
in the tissue samples were determined by LFE Environmental Analysis Laboratories.

Hematological Studies

The following procedures were used to obtain baseline hematological data from

animals collected off-site and in NAEG Intensive Study Areas.

Animals were captured alive utilizing Sherman live traps or nooses and returned
to the laboratory, either CETO or UNLV, and examined within 24 hours of capture.
Blood was obtained from lizards by decapitation without anesthetic and was
obtained from rodents by cardiac puncture using ethyl ether as an anesthetic.
In all instances, blood was collected in heparinized (ammonium sulfate) syringes.
All analyses were done in duplicate, and any test exceeding a 1% difference
-was repeated.
All analyses were done following standard methods (Hepler,

1966).

Differential Stain--Blood smears were prepared on clean slides and allowed

to air dry.
Slides were then fixed with Wright's stain.
One hundred
cells were counted and identified using the 4-field meander method,
beginning at the margin of the smear and counting toward the center and

then to the margin alternately.

Hemoglobin--The cyanmethemoglobin method was used for hemoglobin determination. To 5.0 ml Drabkins solution, 0.02 ml of whole blood was added.

Hemoglobin is converted to cyanmethemoglobin by the Drabkins solution
which contains ferricyanide and cyanide.
The resultant color is indicative

of hemoglobin concentration which was read on a Fisher Flo-thru Hemophoto-

meter in gm/100 ml or Bausch and Lomb Spectronic 20.

Packed Cell Volume--The packed cell volume (PCV) is a measurement of
percentage of red blood corpuscles in whole blood.
Precalibrated 75 mm
heparinized micro-hematocrit tubes were centrifuged in an Adam's Readacrit

Centrifuge at 8500 rpm for five minutes and PCV measured on the built-in
reader (1-100%).

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