SECRET Belated Project .. Study of Hematology of Radiat. on Injury Responsible Agency: National Military Establishment ; ; Objective: To porform certain routine hematoligic oxaminations for comparison with results obtained ot Bikini and in the NMRI, To study the nature and the course of the hemorrhagic tendency in irradiated animals, Proceduret Routinc, complete studies of the hemogram will be made in the surviving large animals, The studies of the homorrhagic state will be confined to histologic examination of appropriato tissucs, and to biochemicnl studics of plasma, ; quick frozen at -55°F, and returned to NMRI for fractionation; Ctle Projoct 7: Syudy of Particulate Fission Products Responsible Agency: University of Rochester Objective: To study the distribution in lungs and cther tissucs of radio:ctive fission products, of dust containing induced radioactivity; using animals exposed at various sites, Procedure: As amended, selected tissues from animals exposed in 3.2.1.1 would be available for preservation for radio- asutography, microincincration, and other suitable studies. (See original proposals 3.22023 3e202e33 3420244; and 3,.2.2,4.1) 502.255 Responsible Agency: National Military Establishment Objective: To study the distribution in animals of fission products inhaled and ingested in areas of fall-out, Procedure: Where fall-out is expected shicldcd laboratory animalswould be expesed for varying intervals of timo; and in known areas of fall-out laboratory animels and indigcnous forms would be collected after vexposure and studied for the distribution and variety of fission products in the lungs and body. UCal* Responsible Agency: Crocker Laboratory, University of Cal ifornia Objective: To investigate the behavior of radicactive acrosols produced by nuclear detonation, using expcriments} animals (rats) to evaluste and estimate the possible hazards to humans inhaling radioactive dusts and mists, Procedure: Animals would be placed downewind, suitably shiclded from bomb radintions, If possible others would be placed in drone aircraft, Spulics of oc: mical species of radioactive aerosols, their distribution, ctc,, would be performed using alpha, beta and gamma counters, cleckroscopces, ctce Project 8: Biological Dosimetry Objoctives: This series of proposals involves the use of certain biological agents os dosimeters, on the basis of extensi ve laboratory studies, Procedures The dosimeter material would be placed in suitable contuinors, and in allthe animal exposure containers, It would also be placed behind experimental shiclds , and in the high dose rangco, It is planned that the dose range: 20-600r would be monitored by Tradescantia; 50-600r by the CF-1 mice; 500-5000r by Neurospora; 5000-20,000r by Aspergillus; and 5000-50,000r by Zen, Responsible Apencies: Tradescantia Zca. Aspergillus * Submitted too late for Onk Ridge National Laboratory, and NME U.S.Department of Agriculture, and NWE Osk Ridge National Laborat ory, and NME Responsible igensy: Los Alamos Scientific Laboratory Objective: To use alternations of the size of spleen, thymus, and changes in the ratio: white pulp/ red pulp after 250 KV X-ray as an indicator of equivalent rodiation. It is proposed to attempt biological monitoring of atom bomb radiation in certain localities by taking advan tage of this effect, Procedurer Mice whose response to 250 KV x=ray is well know would be exposed in the approximately 50-600r range in locations where integrated dose measurements are desired, as behind hiclds, in drones, etc, Approximately 5 days after exposure the animals will be sacrificed and the measu rements made, action by Co:mmittee GC -3