DOCUMENT So, Lawrence Be rkel Chives and Reey‘Labetory cords one ; airl aeae Ae, =ee perer 1? cure about 30°Aof my animals, ‘They turned out sterile, and it was a very careful dosage routine I had to follow. SSH: How were you determining the dosage? KGS: Well, I got a standard from McMillan. McMillan said, "This standard emits 500 aiphe”particles /ory~see,—mst-haue A Seon—bets—partictes—though} per minute, and I built myself a standard out of uranium oxide, which was a powder } wh ant it to discharge the electroscope at the same Tatas)saad Bet See cantanteent 500 erentoene the niera, curides ig. the amount of any isotope, dia@ategration rate of a certain M1 of our radioactive phosphorous was Based on that standard. a SSH: How did you determine biological effectiveness? KGS: By blood counts, mostlyg GaPie they fell out of sight, why, usually the animals would die. Go¥ asa you| find that there was ee localization? Well, tathe bone marrow. GP ghose, Sieys the very beginnings of cadiokostaphyn there—daxe? (Breiner people in the Lab, whose names I've been trying to remember, who were physicists, a (oPrhelpful to, bus A SSH: A I've read something stating that Hamilton was responsible for developing the technique of radioautography. KGS: No, Prieta Neit af ‘We had a Dorothy Axelrod who made nany radioautographs, a Orn: Voith abetly plutoniung/because it*s an alpha matteryA you get en ’ beautiful pictures. : ; the Wd * $38: But that was later, wasn't it? That was thedzmore- RCS: Much, much later, yes. SSH De you know anything about whe was responsible for the initial developuent of the technique? dowe Was that dew at Berkeley?