PRIVACY A\
2
crt METHOD FOR DEEP RAIN ATION OF KET Obi
R. WENNESLAND ET AL.
.
Table V. Cell Volume (Vrbc) at Normal
Kody Temperature and as Found by Injecting a Second Dose of Tagged Cells after
Rectal Temperature Had Fallen to 27-28°C.
Two Patents Subjected to Hypothermia for
Surgical Operation.
ee — a —. te a
-—--
MATERIAL REMOVED
.
Rectal
Subject
Temp.
*c
[Muzi
Tune
min.
Vibe
Hemato-
crie
% calle
a
point. Vrbc was measured in the usua| Wa
before induction of anesthesia and hypothe.
mia. A second dose of tagged cells was Biven
after rectal temperature had fallen abou
10° C. Vibe appeared to be 7-10 per com
lower during hypothermia than just Prior
ne
Table VI. Repeated measurements of cell and whole blood volume in 17 healthy
men at 3 to 3) week entervals
:
Interval inure.
between
measurements
Subject
to its induction. Sunilar results were obtained
in 3 of 4 splenectomized dogs studied during
5
experimental hypothermia.
.q23
First dose
38.0
37.8
6s
55
1.60
41.2
Second dose
274
26.9
26.7
16
2
§2
1.34
1.42
144
446
44.6
4.9
37.7
st
298}
474
of celle
a dose
el
41.0
of cells
37.7
59
2.95
6.4
Second dose
27.0
16.7
15
25
2.68
2.64
50.46
50.6
26.2
$2
2.73
48.2
iously low values (Nylin & Hedlund 1947;
Irown, Hopper, Sampson & Mudrick 1951;
Reilly, French, Lau, Scott & White 1954;
Vriachard ef al. 1955). In cases of congestive
heart failure we take samples at intervals of
15 miuutes for one hour or longer. The resultant “mixing curves” are nearly always
flat by the end of half an hour. Caution is
required in interpreting results from tests
taade during shuck or hypothermia because
there as evidence that portions of the vascular
tree may never be reached by the tagged cells
tu these conditions (Rodbard, Saiki, Malia
& Young 1951; Prentice, Olney, Artz &
Howard 1954). Thus, the mixing. curve may
be flat, but the csr yulyme
distribution represents the cirtulating, father than
the total, cell volume. Dhsedviti
on two
patients subjetted ‘f@ hypothermia before
surgical ofstresfa V) illustrate this
Nom
Jo}
Pork ME
Reproducibility and relative magnitude of
In three experiments, subjects were given
a second dose of tagged cells immediately
alter the first.-Differences between first and
second Vrbc were 0, 90 and 50 mi, or 0, 45
and 3.4 per cent.
Seventeen healthy men were subjected to
second of third tests after intervals of
3-31 weeks in order to avoid using the
latger amounts of radioactivity needed for,
immediately sequential measurements (Taj
ble VI). The mean of 20 differences was’
77 wml of cells, or 3.9 per cent (range, 10190 ml; 0.6—88 per cent). (These ire
define the outer limits of experimental errbr
or beyond, since they are affected not only
by all the compopients of tha method but by
nn
Found
1
472
470
244
225
434
443
wi
vy
1 Ko
11
197
Whole Blood
oe pe
.
|Diflesence] Change
Foursel
[Duillerence|
%
‘
i
v5
vo
j&
5.48
479
0.39
Change
a
15
uu
27
430‘
419
496
u7l
10
41a
01 o
24
349
0 U6
15
on
2. é3
16.
7
4H
1.67
4
4
Los
iol
U.6
5
|. ane
ru
wal
Ys
ae
0.01
03
nol? te
ano
aud
3
a
ral
176
193
30D
vus
24
0.04
11
um
42
7
167
au
4 au
0 20
50
4
‘3?
or
1)
57
ats
024
47
6
4551
45
213
308
vw
42
404
a8
0.16
34
12
“2
1s)
wi
55
“
0.51
11.2
1
"a
\ ‘
put
Ub
ny
ou
77
W
443
448
17x
tHe
wun
45
402
416
O14
3.
469
204
20)
12
en
on
a4
0.09
2.0
this
33
440
404
417
0.13
32
n
component sources of error.
Celle
cm ®
0
.
13
io
tee
:
0.05
win ft
24
ie
455
003
1)
,
possible changes within subjects. Differences
9
458
a4o
452
of lhe :
9
‘oF
af
way
41
in
047
10.0
3
i41
awh
a2
'
2
1k2
178
waz
wy
.
ju
itr
4
ye
023
025
45
gs
6
wi
ang
ba
205
037
v.23
5
6
$.2
O18
41
guitude, however, have beew
found nl bet who repeated measurements
at shorter intervals. Those measuring VCr+#
réported differences of 0.4—10.0 per cent
(Reilly et af. 1954), 4 per cent (Iisenbery
1954) and 2—8 per cent (Walser, Dulfy &
10
:
3,
*
u7
wit
U8
ox
4
wos
23
+44
a7
Gniffith 1956). The experience with P3* has
been similar. Differences Letween paired
Mean
(Chaplin 1954), 3.5—7.5 per cent of cells
® Uncorrected for trapped pkasima sind read te Ure dap of Ure coll coluiom
measurements were 0.1—6.2 per cent ofcells