PRIVACY ACT MATERIAL REMOVED
2
Table V. Cell Volume (Vrbc) at Normal
Hody Temperature and as Found by In-
jecting a Second Dose of Tagged Cells ajter
Rectal Temperature Had Fallen to 27-28°C.
Two Patients Subjected to Hypothermia for
Suryical Operation.
_.
Subject
Tone.
Mixing
1
Vibe
' ct
55
65
1.60
1.61
41.2
41.0
27.4
26.9
26.7
16
32
52
1.4
1.42
1.4
46.6
4646
46.9
First dose
of cells
377
37.7
$1
59
2.95
2.95
47.1
4.4
Second dosa
27.0
26.7
26.2
15
25
62
2.68
2.61
2.73
50.6
50.6
48.2
e
c
.
nin.
First dose
of celle
3&0
37.8
Second dose
a>
i
s
%q cells
iously low values (Nylin & Hedlund 1947;
Krown, Hopper, Sampson & Mudrick 1951;
Reilly, French, Lau, Scott & White 1954;
Prichard et af. 1955). In cases of congestive
heart failure we take samples at intervals of
15 nsinutes fur one hour or longer. The re-
sultant “mixing curves” are nearly always
flat by the end of half an hour. Caution is
required in interpreting results from tests
made during shock or hypothermia because
there 4s evidence that portions of the lee r
tree may never be reached by the tagged cel
in these conditions (Rodbard, Saiki, Mahw
& Young 1951; Prentke, Otney, !Artz &
Howard 1954). Thus, the mixing curve may
be flat, bud the
pliseavéd volume of distribu-
tion repregegts bile fi culating, rather than
the total, ce
‘Observations on two
patients fer to hypothermia before
seri
¥
*
%
%
Meer
F
oa
rocedures (Table V) illustrate this
wh
Sob POR DETERMINATION OF MED CELL VOLUME
&. WENNESLAND ET AL.
point. Vrbe was measured in the us
ual
wa,
hypothe,
ple Vi Repeated measurements of coll and whole bloud volume in 17 healthy
before induction of anesthesia and
mia. A second dose of tagged cells Was na
after rectal temperature had fallen aos
mien at 3 do ST week intervals.
Ta
a
tonereat
10° C. Vrbc appeared to be 7—10 per ¢
ard eh a
yeasure-
subpct
lower during hypothermia than just oie
to its induction. Similar results were obainey
in 3-of 4 splenectomized dogs studied du
ting
experimental hypothermia.
mints
an
s
4
23
Reproducibility and relative magnitude of
component sources of error.
In three experiments, subjects were given
;
a second dose of tagged cells immediate),
;
fl
after the first. Differences between first and
second Vrbc were 0, 90 and 50 ml, or 0,45
and 3.4 per cent.
Seventeen healthy men were subjected to
second or third tests after intervals «
3
it
24
u
Jarger amounts of radioactivity needed for
immediately sequential measuteinents (Ta:
2
ble VI). The mean of 20 differences’ was
7? ml of cells, or 3.9 per cent (range, 10—
10
190 ml; 0.6—8.§ per: cent), These result,
define the outer Ilmits of experimental ersur
possible changes within subjects. Difference,
f\the same magnitude, however, have beeu
Mc
o
472
a7
ava
4u3
aul
aux
avo
421
“O4
any
495
ane
4K
4K
4814
452
456
455
454
2
WO
47
ue
°
a"
452
407
-
-
Change
Fuund
|Differencel
Change
Found
[Difference
I
%o
|
i
2.44
225
019
78
514
479
0.39
25
1ial
Ro
005
2?
On
Oa
26
ws
167
be2
1.66
es
430
419
490
vos
0.04
001
3.0
24
06
0
6
440
‘oc
489
010
005
toe
24
13
e
367
366
a7
3.67
400
420
$09
533
469
453
456
5 07
4.00
io
vo
03
out
1
020
om
50
47
051
on
1.2
402
ate
455
44%
404
47
o.
4s
ow
10
1
197
180
1st
131
176
193
202
2.30
243
23
2.04
183
1493
0.16
0.01
58
O13
28
42
;
5.
0.09
4.2
005
{°°0.09
0.50
35
v.01
06
0u8
45
ou
st
1x2
188
000
33
uw
‘1
var
0
68
163
te
178
ae
214
20)
219
442
22
224
iw
400
ne
142
is
0.07
3
44
415
raz
205
Ot}
43
Whole Hiood
Ceils
447
;
ae
s
Ns
,
ufind by others who repeated measurements +
at’shorter intervals. Those measuring VCr*
feported differences of 0.4—10.0 per ceut
(Keilly ct af. 1954), 4 per cent (Kisenberg
1954) and 2—8 per cent (Walser, Duffy &
“O
ae
“ ee
Hemiato-
443
ae
49
455
3
or beyond, since they ate affected not ouh
by afl the components of the method but by
(Chaplin 1954), 3.5—7.5 per cent of cells
»
4
3
ts
.
S—SI weeks in order to avoid using the
Griffith 1956). The experience with P3® his
been similar. Differences between paired
measurements were 0.1—G.2 per cent of cells
.
a_
227
0 U8
us
9
3.9
ie
O16
7
01)
32
469
047
100
516
512
od
4s
394
te
025
64
434
a7
ov
8S
any
34%
23
® Uncorrected for trapped plisiia aed read to the dop of the ce colunm
0.23
0.18
§2
41