Project Title: 15. Biological Studies of Materials Arising from Energy Operations Interaction of Chemicals with Nucleic Acids Relationship to Other Projects: (Cont‘d.) RX-0 3-06- (b) RX-03-O2-d in collaboration with the Medical Research Council Biophysics Research Unit and Department of Biophysics, King's College, London, as part of a program on the storage and transfer of the genetic message. Others working on the interaction of chemicals with nucleic acids include David Davies at the National Institutes of Health, Bethesda, and H. M. Sobel and S, C, Jane at Departments of Chemistry and Radiation Biology and Bio-Physics, University of Rochester, Several other laboratories are using Nuclear Magnetic Resonance (NMR) to discern the interaction between chemicals and monomeric substituents of nucleic acids, The proposed work will complement proposed studies of D, Borg in the BNL Medical Department on the interaction of chemicals with substituents of nucleic acids and eventually with nucleic acids themselves, to be followed by Electon Paramagnetic Resonance (EPR) spectroscopy and NMR, These studies also relate to studies on research in mutagenesis being carried out by F, J. de Serres at Oak Ridge National Laboratory; on the relation of cancer induction and genetic damage by J. H. Weisberger and G. M. Williams at the National Cancer Institute, NIH, Bethesda; and extrapolating results of toxicity studies in laboratory animals to man by D. P. Rall, National Institute of Environmental Health Sciences, North Carolina, 16, Technical Progress in FY 1973: Project not yet 17. * initiated , - Expected Results in FY 1974: Project to be initiated in FY 1975, 18. Expected Results in FY 1975: The first task will be a survey of carcinogens soluble in body fluids as well as polynuclear hydrocarbons that will require solubilization emitted from fossil-fuel power plants and also present in urban atmosphere, Pure COMP GigR s of chemicals recognized as directly carcinogenic will be complexed in v The } with DNA, various types of RNA, and synthetic polyribonucleotides, feraccion of che carcinogens with nucleic acids will be followed lotometrically, by melting-curve analysis, and when indicated, by ultracentrifugation analysis. In addition, where it is possible to get complexes that remain re-soluble, x-ray diffraction studies will be made on fibers of the complexes, and the data from these combined with molecular model-building of the nucleic acids and the interacting molecules to determine the structure of the three-dimensional interaccion of the complex. [t (See Continuation Sheet) 11719330 RX- 387