Project Title:
14,
Scope:
Effects of Radiation on Living Organisms
Radiosensitivity of Immune Responses and Mechanisms of
Jmmune Reactions _
RX-03-O1-(b)
(Cont'd.)
responses, as compared with the same antigen (tetanus toxoid} administered
either in soluble or adsorbed form,
In order to provide a broad basis for
the observed efficacy of primary immunization with complexed antigens,
three
additional and unrelated antigens, bovine serum albumin (BSA), horseradish
peroxidase (HRP) and glucose oxidase (GOX)
im comparative experiments.
from Aspergillus niger are used
Present findings show a close correlation between an
increased number
of germinal centers in lymph nodes, as well as their earlier appearance and
rapid proliferation of cellular elements, and the appearance of serum antibody.
Germinal centers show both an increase in number and proliferate faster when
mice are immunized with complexes of antigen and antibody as compared with
the same amount of antigen only.
Immune defects in so-called immphologic
deficiency syndromes in man may result from developmental disorders of
immunologically active tissues,
Developmental failure of lymphoid tissues
may result in a functional deficiency, absence of one or more immunoglobulins,
and in morphologically detectable defects.
Although germinal centers contain
immunoglobulins as shown by immunofluorescent methods, it is apparent that
most immunoglobulins lie on the surface of cells or in reticulum cells within
germinal centers.
Present findings with HRP antigen provide evidence for
antibody formation by lymphoid germinal. center cells as well as persistence
of antigen and/or HRP-antibody complexes between dendritic reticular cells. :
Increasing emphasis will be given to primary immunization with antigenantibody complexes prepared with gammaimmunoglobulins (IgG).
Complexes of
tetanus toxoid and specific human IgG have been prepared for primary
immunization studies in man.
The long-range purpose of this research is to |
extend the principles of enhanced immunization demonstrated with the present
complexed antigens to situations where antigens of bacterial, viral, protozoan.
and parasitic origin are poorly antigenic.
Previous work in this laboratory has demonstrated an increased susceptibility of irradiated animals to fatal anaphylactic shock.
Since enhanced
antibody responses are obtained in both normal and irradiated mice immunized
with antigen-antibody complexes, the anaphylactogenic properties of complexes
will be explored when complexes are prepared in antigen excess, equivalence,
and antibody excess,
The scope of this research has been enlarged to include a study of the
In collaborcontrol of antibody responses in many strains of mice.
genegite
atiogswith the Jackson Laboratory, Bar Harbor, Maine, more than 140 strains of
miceSiteh known genetic constitutions are being made available at no expense.
The retative capacity of these various strains of mice to produce primary and
secondary tetanus antitoxin responses is being tested.
When significant
differences in antibody responses are observed, The Jackson Laboratory will
develop new recombinant inbred lines for immunologic studies.
Immunogenetics
has a direct bearing on the genetic control of the gene locus involved in the
(See Continuation Sheet)
1119269
RX-176