Table 6. . ; 90 . . , Chemistry scheme for determination of Sr and Pu in coralline soils, vegetation, and animal tissue. Dissolution Fire coral, vegetation, or animal tissue at 950°C for 8h. Add ash to Y carrier and 242pu tracer.? Dissolve with 12M HCl + 5.5M HI.> Add HNO.,; boil to oxidize Pu, convert to Cl”. Separation Load on Dowex 1-X8 column from 12M HCl (Pu-Y separation). Wash column with 12M HCl. (Load and wash to Y purification.) Elute Pu with 12M HCl + saturated NH,I (to Pu purification). ¥ Purification Precipitate Y(OH), by adding NH,OH. (Note Sr-Y separation time. ) Wash precipitate with H)0; dissolve with 16M HNO3; dilute with H90- Precipitate Y(OH)3 by adding NH,OH; wash precipitate with H»O. Dissolve in 0.1M HCl. Extract twice with 10% HDEHPS in toluene. Back-extract with 3M HCl. Precipitate ¥ (OH) 3 by adding NH,OH. Wash with H90, dissolve with 12M HCl + H50, filter. Precipitate Y oxalate by adding saturated oxalic acid, digest. Filter precipitate, dry, fire to Y903 at 900°C, 2h. Weigh, count 90y betas. Pu Purification To column eluant add 5M NH»OH-HC1, LaCl, carrier, saturated NH,I, Zxr0(NO3) 5 Carrier. Precipitate LaF3 by adding HF. Dissolve with HNO, + H4B03. Precipitate La(OH) 3 by adding NH,OH. Dissolve with 16M HNO3, boil. Precipitate La(OH)3 by adding NH,OH. Wash with H»0; dissolve with 12M HCl + few drops HNO 3. Load on Dowex 1-X8 column. Wash with 12M HCL, 12M HCl-dilute HF, more 12M HCl. Elute Pu with 12M HCl-saturated NH,I. a. Add 12 drops H9S0,; fume to SO 3 evolution. Transfer to plating cell; electroplate (for Pu determina~ tion by a-pulse-height analysis). OR b. a 2 Transfer to mass spectrometry for filament loading (for Pu determination by mass spectrometric analysis). 425, was used as a tracer for pulse-height analysis (a-PHA) and for mass spectrometry. Note that 238 py could be determined only in those samples that were assayed via a@-PHA. The addition of HI is necessary to insure equilibration of plutonium tracer with the plutonium in the working solution. Bis(2-ethylhexyl)orthophosphoric acid. -l1= as 59010053