-7Procedures Used in Collecting and Processing Samples and Recording
Radioactivity

The procedures used in cullecting samples of biological material in the
Marshall Islands have evolved over a period of years.

An effort was made

during each expedition to obtain as complete a sample as necessary for an
adequate evaluation of the problems studied, without completely swamping

the Laboratory with samples.
To carry out the program of sampling, specific animals and plants with
wide distribution have been selected for study.

From these selected samples

certain tissues are evaluated to determine the distribution of radioactivity.
Collections made in the field were retained on ice or frozen until they
could be returned to the Division of Biology and Medicine field laboratory
at Parry Island.

There the or:anisms were identified, selected tissues

were dissected, weighed and then dried.

The packaged dried samples, to-

gether with the data cards, were sent by airmail tu the Applied Fisheries
Laboratory, University of Washington,for further processing.
At the Applied Fisheries Laboratory, the dried samples were ashed at
temperatures up to 540°C, cooled, slurried, dried, and then counted in an

internal gas-flow counting chamber.

The counts per plate were converted

to disintegrations per minute per gram (d/m/g) of wet tissue, as of the
date of collection, by correcting for sample weight, geometry, backscatter,
self-absorption, coincidence, and decay.

of these procedures see WT-616 7

For a more cumplete discussion

Select target paragraph3