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The transition elements, nickel,
iron and zinc,were
determined
described by Sandell (1959).
rated.
manganese,
cobalt,
colorimetrically by
The elements were initially sepa-
Kraus and Moore
(1953) have shown that
the chloride complexes of the transition elements,
are adsorbed onto a
(Dowex 1)
HCl.
methods
by the selective elution of their chloride complexes from
an anion exchange resin.
zinc,
copper,
strongly basic
nickel through
anion exchange resin
and are selectively eluted at different molarities of
Following the same principle,
Joyner and Chakravarti (1960)
have suggested techniques which were applied to these samples.
Light transmission of the solutions containing each of the
separated icns was compared against sets
of standards with
a
Bausch and Lomb "Spectronic 20" adjusted to specific wave lengths.
Protein nitrogen was determined by the
Kjeldahl method.
Fat was determined by a modification of the Johnson method
(Winton and Winton,
1945).
‘Methylene chloride was the extracting
solvent.
Ash content was determined as the nitrate form by drying
an aliquot of the ashed sample to constant weight.
The moisture content of the sample was calculated from the
wet weight to dry weight ratios.
The total carbohydrate and like substances were estimated
by subtracting the moisture,
fat,
protein and ash from the total