10
Table 6.
Mitotically Connected Abnormalities
dicentric chromosomes and monocentric abnormal
(per 1,000 Erythroblasts)
chromosomes,
Subject IBinucleated
Xo
Erbl.
om
T-3:
4
K—-1/)
33
T-8.
kK-2/')
‘Karyomere
|
+:
oO
K— 4,
K—5
067
13!
K-38,
K-9
0
2
K-ll
4
K~6,
K-10:
K-12.
K-13:
K-16
Normal
1
2
3
4
5
i
13.
13
0.67
2
27
2
4
0
0
2
2
572
13
+
i
{
;
Mitosis |
0.67
0
7
OS
2
11.3
0
9
0
0.67
067
4
"9
0
0
0
Giant
some in "Neutrophils
"1b
.-
4
Cperrant
+
0
>:
0
_
=
_
_
—
2
13
0
_
0
4
_
~
0.67
+
1.3
7
0
0
_
_
13
2
0
0
0
0
_
:
chromosome-type
which have never been observed in normal
persons, occurred in them. Dicentrics were found
in 7 cases, and monocentric abnormal chromo-
somes were present in all of the cases examined.
Also, aneuploid cells occurred in a high frequency.
In view of the importance of these findings,
long term observations are to be made. The
chromosome abnormalities of these patients were
reported elsewhere together with those of other
radiation-exposed people, i.e. atomic bomb survivors, Thorotrast-injected patients, etc.'?
G. Other Laboratory Studies
Several laboratory studies were made on the
subjects. The results of these examinations were
mostly within normal range.
Normal.
_
Thymol turbidity test:
_
_
_
Icteric index:
_
Cases K-5, 6,8 and 9 revealed high values (7~10).
F. Chromosome Studies
Chromosome studies succeeded on 13 of the
exposed persons. The observation of chromosomes was made by cultures of peripheral leucocytes prepared in accordance with the method by
Moorhead, et al. with a slight modification.
Samples were obtained 72 hours after initiation
of culture, and 200 metaphases were examined
in each culture for all the cases except one
case. The control observations were based on 11
normal males, aged 28-53. The results of the
observations are shown in Table 7.
As shown in the table, frequencies of chromosome aberrations in the exposed persons were
much higher than those in the normal. Further,
Cases K-8, 11, 13 and 16 show-
ed slight increase (nearly 9).
Serum alkaline phosphatase:
Cases K-6, 7, 10 and 11 indicated increased values (12~18 King-Armstrong
In the histological sections, all showed nor-
malities were found.
However, some
of them indicated slight abnormalities.
ZIT:
mal cellular marrow.
c. Haemorrhagic diathesis
As to the haemorrhagic diathesis, no abnor-
abnormalities
unit).
GOT:
No
pathological
data
was obtained
except for T-7 (70 Frankel unit).
GPT: All data were within normal range.
Paper electro phoresis: No abnormalfindings were
obtained.
Total cholesterol: All were normal.
BSP test: Cases T~6 (12.5%), K-6 (5-7.5%) and
K-13 (5-7.59) revealed slight high
retention (45 min.).
Erythrocytes sedimentation rate:
All were normal.
Tests for syphylis: All were negative.
Routine urine analyses:
A sample from Case T-7 was positive
Feces;
for sugar (1/10~1/494). Others were
normal.
No parasites were observed.
PBI:
Five cases were measured for PBI con-
tent by courtesy of Dr. Tachibana (St.