Table 2
Fission Track Autoradiography Procedure

used on two

teeth samples

from rhe

Marshall Islands

bescription of teeth:

Step
Dehydration

Infiltration

Embedding

no ID
Tooth A was an intact incisor
Tooth B was a molar with very little of the roots attached
Comments

Description
2 to 3 changes
ethanol

in absolute

placed in unpolymerize

methyl methacrylate +
IZ (by weight) of catalyst,
benzoyl peroxide for 2 days
replacement of above by
methyl methacrylate +
accelerator (25:1 by volume)

To minimize outgassing when teeth
are being irradiated and to permit
the polymer to infiltrate the teeth
full infiltration of teeth is more
difficult than of bone

The accelerator causes

the polymeri

-zation of the methyl methacrylate
through an exothermic

reaction

Curing

allowed the sample to solidi- Better curing can be done by using
a pressure chamber (e.g. 250 Lb/in
fy in a vacuum dessicator
and 50 °c)
overnight

Sectioning

use of carborundum saw

to remove parts of glass
mold

‘Ten sections ranging from
356 un Co

L816 um in

thick-

ness were obtained from

The cured material stuck to the.
glass mold.
Such should not have
occured if a mold releaser is
used.
Thinnest section obtained of
encapsulated teeth had been 250 yim,
according to the instruction manual

the two teeth samples with
a macrotome 2? precision
saw( 100 mm diameter diamond

blade, water cooled)

Preparation
for irradiafion

Each section was sandwiched
between lexan foils (250 um

tlric’ ,

&5 cm diameter) &

Wrapping oyAl foil is supposed
to provide OFact between teeth
and Lexan

5 pairs from cach teeth
stacked one after the other
and wrapped in pure Al foil
[rradiation

10+ at /em® see neutron flux

ratio is desired
A low n
prevent g 245B — fission and

for 2 hes at the Medical
Research Reactor which has
a thermal to Last neutron
Ambient
ratio of about 33.
Lemperature in the capsule

Control

is preater

diateq batch

5

than

50%

The

TaL(n., &) 24

reaction

teeth were inaccessible
Control lexan
at the time.
was not included in the irrathis

time( plain

to

Select target paragraph3