22 It is proposed to show that these mechanisms, operating selectively between the dissolved and solid phases of sea water, can bring about an apparent discrimination, ecological system, in a marine between ‘isotopes of the same heavy element. The accumulation of zinc ions by fish liver cells against an apparent 7-fold concentration gradient is considered by Saltman and Boroughs (1960), to be a passive mechanism not Girectly coupled to metabolic energy, involving sorption of zine to binding sites on or within the cells. The binding sites were not specifically identified but were shown to be profoundly altered by changes of temperature, pH and chemical environment. Black and Mitchell (1952) analyzed spectrographically some of the common brown algae (Laminariaceae and Fucaceae) and sea water samples from the Scottish coast, reporting con~ centration factors of over 1000 for some trace elements, nota~ bly Ti and Zn, in these algae. Lagrange and Tchakirian (1939), using spectrographic methods in analyses of Lithothamnium, a common reef builder, detected 15 trace elements in addition to the major constitu- ents. Chaberek and Martell (1959), cite the outer surface of vertebrate bone as an ion exchange medium capable of adsorbing

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