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November 30, 1962
Leo M. Meyer, M.D.
South Nassau Communities Hospital
2445 Oceanside Road
Oceanside, New York
Dear Leo,
Here is the procedure on the use of tritiated water for
measuring total body water.
1.
We will provide the HTO,
Let me know by return mail if ve
should send it to you and where, or if you will pick it up when
you pass through San Francisco,
2.
Give by youth 1 ec of ATO.
Transfer from stock bottle to
a glass, cup, or beaker exactly 1.00 cc HTO with a tuberculin
syringe (or 1 ce pipette).
Add 50-100 ce water and have sub-
ject drink entire contents.
Add another 50-100 cc water to the
vessel and again have subject drink entire contents.
3.
Tritium dilution can be determined in either plasma or urine
(or both).
If urine samples are collected, follow this procedure:
(a)
Have subject vold about & hrs. after taking tritium. (b)
Take 10 cc uPine samples at approximately 5 hrs., 8 hrs., and
12 hrs. aftertaking tritium,
can be obtained.
A 2+ hr. sample is useful if tt
Put the urine sample in a 3 or 5 dram vial
with a erystal of thymol and labeled with name or code number,
date and time after administration of HTO.
If a plasma sample is taken (in addition to or instead of
urine), take the sample % to 6 hrs. after giving the tritium.
Lawrence Bertoley Laboratory
Archives and Reserds Otheo
I0082b
Newt
PPIIED SCIEACE Div.
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