ll
the UeSeSe Chilton yielded 144 fish.
These fish were used to provide natertal
forcactimity counts and because of their availability near laboratory spaces,
“they were also used to provide tissues for future study.
The collections of the more sedentary forms, such as clams, sea urchins,
sea cucumbers, snails, coral, and algae, were usually made in the intertidal
zone or water shallow enough for skin or shallow water divers to penetrate,
Preservation of material,
An attempt was made to use as much of the material as possible while
2a
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still in a fresh condition.
Thermos jugs with ice were carried to the
collecting areas and organisms to be used fresh for activity counts placed
in these jugs for transportation back to the laboratories on the Chiltone
Both a refrigerator and a freezer were provided aboard the Chilton for
temporary storage of material.
Of the total of 5883 organisms collected (Table 1) 735 were used
fresh for measuring activity (Table 2) and 5148 were preserved in alcohol or
formalin for shipment to laboratories on the mainland for further study,
Ashing and activity counting.
Specimens to be counted were selected in the field at the scene of the
collection and immediately placed on ice in thermos jugs to slow down decay.
This could not be done with large fish caught on hook and line.
An attempt
was made to select species which were likely to be found at all sampling
stations agains
The following series of tissues were dissected from the fish so far as
was practical: bone; gills; skin, including scales; muscle; faeces; a section
of the intestine; liver; spleen; gonads; and kidney,
selected from some of the invertebrates,
brates were used in, their entirety.
Similar tissues were
Many of the small fish and inverte=
~ UNIVERS IY
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