Long~line gear was fished at 17 stations during the four cruises. Sixty "baskets" of 6-hook, 180 to 210-fathom mainline gear with 16-fathom droppers were fished at each station. The catch rate ranged from 0.6 to 21.9 tuna per 100 hooks per set. Details of the catch are given in Table 2. Some of the skipjack tuna used for samples were taken by live-bait fishing, whereas the flying fish samples were obtained from specimens that flew onto the ship's deck at night. Samples of muscle and liver were dissected from the fresh fish, labelled, placed in a plastic bag, for transport to Christmas Island. and frozen aboard ship Here some samples were weighed, dried in an oven at 95° C for 24 hours, reweighed, ground and placed in a standard size plastic container for counting; other samples were frozen or dried and shipped to the Laboratory in Seattle for further processing and analysis. In most of the tuna-like fishes samples of light and dark muscle and liver were analyzed separately. Plankton and nekton samples were collected with one-meter diameter plankton nets and a six-foot diameter Isaacs~Kidd midwater trawl. Sixteen 30-minute surface plankton tows, four 30-minute, thirty- 50-meter plankton tows and one 30-minute, 100- meter plankton tow were made with the one-meter open net. Settling volumes, after the large organisms fish, etc.) were removed, (jellyfish, lantern ranged from 73 to 1,150 ml per tow.

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