32 Tabie 16 Immunoelectrophoretic Analyses Subject Nos. Findings Normal 15, 30, 20, 78, 8, 76, 92, 77, 9, 14, 33, 16, 2, 21, 57, 47, 4, 37, 10, 73, 12, 914, 835, 838, 875, 844, 865, 896, 982, 836, 832, 936, 955, 956, 957, 830, 960, 939, 893, 979, 840, 833, 915, 967, 975, 882, 898, 1005, 868, 822, 1042, 841, 825, 885 Slightly increased y globulin precipitation-line 53, 71, 86, 52, 63, 22, 32, 87, 59, 54, 79, 6, 66, 1, 45, 928, 856, 883, 940, 1036, 944, 992, 858, 813, 864, 853, 887, 942, 829, 820, 828, 924, 961, 969, 895, 855, 884 Markedly increased y globulin precipitation-line 862, 891 Slightly increased precipitation-lines for 6,,, 4,,,, and y 34, 75, 27, 817, 998, 888, 814 Markedly increased precipitation-lines for §.,, &,,,, and y 64, 958, 1035 Markedly increased precipitation-lines for 8,,, and y 829, 892, 1001 Slightly increased precipitation-lines for 8,, and y 3, 41 Markedly increased precipitation-lines for 6,, and y 897 Slightly decreased precipitation-line for 8,,, 5, 900 Slightly increased precipitation-line for Boy 29, 852 Decrease of 8,, and slight increase of y globulin precipitation-lines 866 Decrease of 8,, and y globulin precipitation-lines 60, 68, 824 Glucose-6-phosphate Dehydrogenase Activity and Hemoglobin Types Dr. Boyer at Johns Hopkins Hospital reported that all subjects examined had normal glucose-6- phosphate dehydrogenase activity. On the starch gel electrophoresis all were of a uniform glucose-6phosphate dehydrogenase electrophoretic type Class B. This is the type observed in all Americans of European ancestry and in 70% of Americansof mixed African and European ancestry. Electrophoretic studies of hemoglobin showed that all Marshallese subjects examined had type AA». Blood Volume Studies During the 1961 and 1962 surveys, blood vol- ume determinations, with use of Cr*?-labeled sodi- um chromate, were performed on 25 normal Micronesian and Caucasian persons living in the Marshall Islands for 1 yr or longer. Table 15 shows the data for 23 of these on red cell mass and plasma volume based on body weight. From these data it appears that there was a significant reduction in red cell mass and/or plasma volume in 15 of 23 subjects, both Marshailese and Americans. During the anticipated 1963 survey, it is planned to repeat these studies in conjunction with estimations of lean body mass by useof tritiated water. Evaluation of the above results will be withheld until completion of the 1963 survey. immunoelectrophoretic Studies Immunoelectrophoretic analyses were carried out on a number of Marshallese sera. These results are shown in Table 16. Dr. R. Biitler, who carried out these analyses, reported that “in summary we have foundneither a paraproteinemia nor a typical picture of antibody-deficiency-syndrome. The high frequencyof increases of some of the immunoglob- ulins is perhaps a typical sign of the investigated population.”

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