SOFT TISSUE (WET FROZEN OR DAY) CUT INTO SMALL PIECES 250-g PORTION IN 2-1 PLASTIC BOTTLE HNO3, HF, CAP LOOSELY (CAUTION) ZN WATER BATH TO REACTION. SUBSIDENCE REACTION MIXTURE H3803,4 20MIN, TRANSFER TO BREAKER, BOIL DOWN, COOL SOLUTION + FAT + HNO3 + H3BO3 FILTER DISCARD OR PROCESS OPTION vy FAT . ASH 425°C HF, HNO3z - H3803 DISS. DISSOLVED lO0O-g SAMPLE soln a. ALIQUOT FOR TU ANALYSIS b. STORE WITH VENTED CAP. (CAUTION) FIGURE 1. WET DISSOLUTION OF SOFT TISSUE 279