reported mean soluble nitrate content
ppm; whereas, a maximum of 15 ppm was
is also well established that burrows
content than typical desert soils and
and more favorable for living systems
and Yousef, 1972).

in regions of two burrows at 221 to 570
found in surrounding desert soils. It
are much higher in oxygen and water
temperatures in burrows are more stable
than those found in open desert (Bradley

The animal burrow, then, is a different environment than that found in the
Soluble nutrients, gases, humidity, and temperatures are
surrounding desert.
conducive for maintenance and growth of organisms.
more
are
which
at levels

Burrowing activity may significantly influence distribution and resuspension
of soil components, including probably Pu and Am.

It is important to realize that time spent in burrows by small vertebrates and

many invertebrates is significant and, in many cases, is greater than that
Nonhibernating rodents spend approximately 85%
spent in aboveground activity.

of their active lifetime in burrows.

For hibernating species (P.

longimembris,

for example), burrow occupancy increases to approximately 90% of an average

year (Bradley, unpublished data).

We estimate that lizards may utilize hiber-

nals for approximately 30-40% of an average year and inhabit nocturnal retreats

throughout their activity season. Food stored in animal burrows provides an
ideal substrate for the growth of fungi and bacteria and also a convenient
food source for many invertebrates.

Au and Beckert (1975) have reported that

Aspergillus niger, an ubiquitous soil fungus, can take up several chemical
forms of Pu, including plutonium dioxide, from a cultural medium and have

reported on microbial numbers and relative abundance of fungi in surface and

subsurface soils of NTS.

From analyses of stomach samples, Thomas (1975)

reported that darkling beetles (Tenebrionidae) consume large quantities of

fungi, particularly Aspergillus.

In a preliminary effort to estimate tissue

burdens in some possible food sources of small vertebrates, darkling beetles
were collected from GZ of Area 11-C, washed with alcohol to attempt to remove
surface contamination, and gamma scanned by REECo personnel.
Plutonium tissue

burdens were estimated at 0.5 nCi/g ash.

Burrow microenvironments may differ radiochemically from the soil surface and

may provide significantly different sources of contamination by inhalation and
ingestion.
Therefore, Pu and Am concentrations in burrows and hibernals may
show higher correlations with tissue concentrations than activity strata

developed from surface soil samples. Laboratory error may account for some of
the variability in samples, particularly the homogenate problem already dis-

cussed.
It is obvious, however, that before more conclusive statements can be
made, more data on radiochemical and biological inventories of microhabitats,
such as burrows, and food sources, of some vertebrates have to be available

for evaluation. These data may be critical in order to properly evaluate Pu
and Am uptake by inhalation and ingestion. Radioisotopes in these sources of
contamination may differ in relative concentrations, physical state, and
solubility from those of soil samples analyzed to date.
Resident Rodent Populations of Area 11-C

One particularly difficult problem in evaluating tissue burdens of small
vertebrates occupying different trophic levels and inhabiting defined activity

204

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