“ oa§ a aerb. ~ UNCLASSIFIED: “ae : : ware ps ANALYTICAL PROCEDURE FOR DETERMINATION oF NORMAL STRONTIIM IN BONE 1. Ash the bone sample in a nickel crucible at 900°C, 2. Grind in a mortar to a fine powder. 3. Weigh ont 5 grams into a 250 al centrifuge bottle. U. Add Ls ml of water and then slowly add Lyewlt 90% ‘ 7 itric acid to bring. concentration to“158 . 7 5. ‘Kad 20 ng’ of Bat carrier (as BaCly) in 2 ml of solution. | 6. Star. rapidly for thirty minutes (mechanically). Te ‘Centrifuge for tan minutes at about 2000 Tepe 8. Desant and repeat steps ) thru 7 at V2 the originalvol 4 ’ | Z| : Y 7 - § ; = 9. Decant as much of the liquid as possible and transfer to 100 ml beaker with water. 10. Ewaporate to dryness and pick up with 10 flof concentra Tditric acid. Li. “Add 5 ml of 1000 ppm Li solution, dilute to 100 al and by fleme photometry. eto There is no agreement in the literature ag to the strontium cd@ntent of the normal human being. In part this is due to the variabjlity of available strontium in different sections of the country of the world, and in part the disagreement is due to the methods of Enaly- To resolve the differences for our own laboratory studies, a Rest sis used. was made of two uethods of analy#is. Tie did not permit a lete program of sampling and analysis of bones from several individuals in several localities to be made. ee O3 UNC LASS IFIED re th a UNG ! 2 (9 i.Lietsigpel gd 00131986 055 1031084 At