990
SHORE ET AL.
Table 3
IN VIVO RELATIVE INCORPORATION OF “C— Phe INTO 4C- Phe
A.LF, OF FETUSES FROM STARVED SHAM- AND X-IRRADIATED RATS
g
{c)
Treatment
Starved and
sham
irradiated
Relative in-
“3
corporation
(b)
x
1077 dis/min/g fetus
of 4C— Phe
Number
fetal
(a)
fe)
into MC Phe
(a)
per
weight, “C-Phe "C—Phe
(Nf
ALF,
Litter Iltter
mg
A.LF,
A.S.F. {d) + (e)
(a/(h
R
§
12
12
158
137
28.8
26.8
§5.6
1.4
84.4
78.2
0.341
0.343
u
v
Ww
x
Y¥
10
14
il
il
14
131
135
135
154s:
121
.
27,3
19.9
23,0
30.3
20,7
64.9
36.7
40.1
44,2
39.8
82.2
56.6
63.1
74.5
60.3
0.332
0.352
0.365
0.407
0,343
T
12
144
30,8
57.6
88.4
0.348
X
S.E,
Starved and
irradiated
z
AA
ll
13
125
104
21.1
24.0
AG
13
136
26.4
AB
AC
AD
AE
AF
14
15
12
12
li
116
118
103
120
120
BEST AVAILABLE COPY
22.9
24.7
18,3
21.6
11.4
49.5
§1.8
70.6
75,5
46,5
72.9
46.5
49.5
38,0
45.8
27.6
0,354
0,010
0.299
0.317
69.4
74,2
§6,3
67,4
39,0
0.330
0,333
0,325
0,320
0.292
0,362
Be Roh
X
0,322
analysis of variance of the data in Tables 2 and 3 showed statistically
significant effects due to both X radiation (p < 0.05) and food and water
deprivation (p < 0.05). No indication of interaction between these two
factors was detected; thus the effects are additive.
The multiple-range test!® indicated that incorporation in the starved
and sham-irradiated and starved and X irradiated groups was significantly less than incorporation in the respective fed groups (p < 0.05).
Further, it indicated that “C—Phe incorporation in the starved ir-
radiated group was significantly lower than the incorporation observed
in any of the other experimental groups (p< 0.05). Thus, these data
indicate that, in the fetus present in its intrauterine environment, X
radiation causes a small but s{gnificant decrease in the incorporation
of “C—Pheinto protein per unit weight of fetal tissue.
Irradiated fetuses increased in weight from day 13 to day 14 to
the extent of only 72% of the increase observed in the unirradiated
fetuses. Thus, expressed in terms of relative incorporation per whole
fetus (g xc, see Tables 2 and 3), the differences were greater than the
differences in relative incorporation per unit weight of fetal tissue.
Table 4 presents data on the incorporation of '!C—Pheinto the
A.LF, in fetal cell-free systems. Since precursor {C—Phe was main-
t
i