* Att. C: Proposal* Number Sel THE PROGRAM OF TESTS APPROV 3 BY THE AD HOC COMMITTEE Project 1: Animol Colony Responsible Agency: National Military Establishment (NMRI) Objective: To provide an adequate number of micc, dogs and pigs. Theso onimals would be acclimatized to the total environment, Their LDsq for 250 KV xernay would be determined aftor acclimatization, It is planned to provide 5,000 ~ 10,000 mice of the CF-1 strain; 500 dogs, preforably the Rochester boagle strain; and 500 pigs of the U. 5. Department of Agricul- ture "hairless" strain, Procedure: If approved, oa Noval party will ostablish the colony ae once to permit approximatcly 18 months of breeding. A veterinarion, indoctrinated in genetics, will accompany the party. Now animals will not be introduecd into the colony later than one year before the expected date of the tests, Sufficient animals will be bred to provide adequate controls, A resorvo colony of equal size will be developed at the NMRI, After the tests, representatives of the in caso of accidents, species, and survivors of the tests will be returned to NMRI for furthor study. Project 2: 3.2.1.1 Study of Acute Radiction Injury Responsible Agency: National Military Establishment Objective: To cxpose cnough animals in enough positions to obtain valid statistical data for a study of acute lethality, vs distance «nd dose; LDs&q vs distance and doscs and survival vs distance «nd dose. Procedure: Mice, dogs and pigs will be placcd in containers suitably shiclded from heat and blast, These will be placed in the water end on land at predetermined distances from zero point; and are to be located so that they may be rotrieved promptly, Animals will then be observed, sacrificed scrinlly,etc, 5201.4 Responsible agency: «argonne National Laboratory Objective: To attempt to separate the effects of prompt nuclear gamme radiation, neutron radiation, and gamma radintion from tho firebnll, Procedure: Mice will bo placed in heavily shicldcd containers with collimating slits directed toward the detonation point, and toward the estimated location of the fireball, Acute lethality, LDsq and survival will be studied. 542.461 Responsible Agency: National Military Establishment Objective: To obtain animal tissues aut the carliest possible time after cxposure, and serially thereaftor,for deotuiled histologic and hematologic study. There is a lack of information from Japan and Bikini on this carly period. Procedure: Animals exposed as in 3,2.l.lwill be retrieved at once, Some will be sacrificed at onee, others serially at appropriate intervals thereaftor. The tissucs would be fixed and preserved for study in the Naval Medical Research Institute, the Army Mcdienl Center, and the army Institute of Pathology. Blood studics would be made in the Island Laboratory. Se 204.8 Responsible agency: Natio. Military Establishment Objective: To obtain animal tissucs at the carliest possible time after irradiation, and scrielly thereafter for histochemical studics. Such studics are being performed on tissues irradinted by conventional means, but none have been done on tissues exposed to atom bomb radiation. Procedure: Tissues as obtained in 3.2.1.1 ond 3.2.4.1 will be quick frozen at ~55°F, and stored and transported in this condition to the «irmy Medical Center for histochemical study. Se 264.4 Responsible .igency: National Military Establishment Objective: To obtain animal tissues at the earliest possible time after 2eas sorially thereafter for studies of tissue enz; Such studies have been performed *Refers to Agenda with xeradicttion; none with the intense atom bomb radiation.