AOLPOL
Cancun March-April 1956
272
Vol. 9
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j
BEST copy) AlaILaBLe
A
BLOOD-VOLUME MEASUREMENTS IN CANCER USING
THE Ce®! RED-BLOOD-CELL ‘VAGGING METHOD
W. A. Reicry, Mp, Wasoip 1. Heewic, vH.p., AND KENNEDH G. SCOTT, PH.D.
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tent and its possible uigrificance in relation to the: ! 4,
aetiology of the discage.
io
URING « selics Of tumorhost studies in
D rats and wnice, we howd that the animals
develuped anemia ielatively soon alter im-
plantation of the tumor. “Phis association be-
METHOD
wef 1
Animal Studies, Blood-volume determina:
tions were made on twenty-two control and
iween inaliguaud tumor and anemia has been
on twenty-nine tumor-bearing Slonaker rats.
observed previously by a muuber of investiga- The tumor ued was 4 transmissible fibrosartors, a3 cited by Ewing in 1940. Later studies coma that has been described.’ Subcutaneous
by Sandberg et al. seported the occurrence of inugralis were wade by bilateral inoculation
a sevele secondary anemia in n¢aplastic div of a suspension of tumor cells. Inoculation of
ae. Strong ail Francis have reported phat this particular unmor has produced a high
stains of imice susceptible ig cumore had
reeniage ol takew The resultant tumor
lower hemoglobin levels ian nonsusceptible
lave never been observed to regress but to
inice.* They reported alse that the hemuglabin
grow rapidly and overwhelm the host in a
level progrespively decreased with age in sus period of trom three to four weeks.
cepuible mi g¥ In a report on the iclauionahip
‘The Cr®! used in these studies was produced
of tumor prowils aud hemoglobin level, Tayin the cyclouen by bombarding a vanadium —
for and Pollack comchaded thar the decrease
target with protons to furcarrier-(ree radioHt hemoglobin ts a direct, vather than a sec
chromium as a result of the p,u reaction. In
obdary, cHeet of the waulignant Gamur.
sOHe Casts, niOgrand quantities of sodium
Aseiies of blood volume measurements was
Mitiated On laaa-bearing tal in order ta chiomate carrier were added to ¢lfect a more
sulistactory tapping of che ved blood cells. The
deternmne wheiber the observed ancmia was
uptake of Cr*) by red celly was found to be
the result al execssive red cell destrmetion of of
less when canieriree Cre! was used. lt was
ak eexpansivn ab plisina valuime. Because of
the relatively senall redcell nas ob the rat, suspected that the minute quantities ol hemoglobin present in the saline in which the cells
determiiations were niule by the method of
were sippcnded sequesicaed the small amount
Guay uid Sterling? + which atilizes Cast as
2ted blood ccll bag to aneasute the cincub iting
ob anicrhee 1S) dns pking it unavailable
Woe i ww became aadlable commercially
asa pray standard. \ knowa volume of the -
Jon dithusion inte the real tls.
At aliquot of the tagged cells was set aside |
voc tiiss. AD a later date, when Cie) with
asaithacnily high specie activity to was rane
tugged cells, WE to 0.2 cc, was then adminis-
(Abbou Laboratories), ineasaements on pit
tently WH Sandans types of caameer were inGlided in the expanded progam, Che value
OE Ci ity a hike aad ia estimatiog the red:
Cell tnis aad whole blood volume tas been
feportedd by ws earlion®
tered to normal und cuinor-Learing Tats via
the extevial pupulas vein, which was exposed
by 4 small incision in ibe skin lying directly
over the clavicle. A imixing time of thirty |
minutes was allowed and a sample of cardiac blow, Lio 4c, was withdrawn. Each sample
was Counted on ad scintillation counter, using
@ thallimm-activated sodium iodide crysal in
conjunction with a $819 R.C.A. photomultiplier tube. Phe efficiency of the sciucitlat on
fiom the Roelitsatope baad, Velen AdmuusistyWon Hospital chow Miley), and the Depantinent af
Radiology, Univeasity of Calthamia School of Medicine,
Sate Fiatieiocss, (adda aia
Diits Wonk wars steppretecd dae
posed Tey ot pean disomy
the Steusy, baie, vad fieae D Deenbou Fund ab the
Aciican Caner Society, tatitusnia Davies.
We aie nidebqed te Map. J
Howe, wink Mie TL
No
counter was such thac El to 14 per cent of the
Katina rays ciiitted by the Crt were registered as counts. The sainples were counted
Castle, Mr. Chaules
| fobosen fur thei: assbtance in
Gnnlaciiag the ctinacal adultes,
45) bee ad ane clicmiiin was made wtailable to
fon iw sutheienc period of time to reduce any
Vaiiation awit to statistical fluctuation tw
percent,
us hy Lr. Veeyls G.. Hlamaton, Oirecta of she Couches
lalatory, Univcaiy of Calor, Weikeley, Caliturin.
Reeve fia publiaation, August 19, 1955.
“Vhe blood volume was calculated by cam.
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