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XeRAY TNOUCRDCH®CHSSUIE DAMAGE IR Ma’.
AO4aG FO
Pvidence thot Xerays nn\ othar types of nuclear and allied
radiation gause ehromosema danace lag been fortheoming froa many
years and hae acowmilated from th. study of a variaty of plant and
animal tissues (Lea 1956).
From those studies it head vee
generally sesuned that the proiuetion of chromosome damage in man
wae an important sechanisea in the establishment of the acute
@liniosl effecte of radiation exposure, end possibly aleo in the
idee
@augation of guch delayed effects as the inivetion of leuknesia
(Court~-Brown and Doll, 1960).
Aq fer ae direct observations on human tissues are concerned,
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of bone marrog, noted evidence of chromesone ‘stiokiness’, elumping
(2
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Fliedner and his colleagues (1959), using simple squash preparations
of chroscacues and anaphase bridge formation in a number of persons
accidentally exposed to a mixed neutron-gemsa ray beam,
Bender (1957),
Peek and his colleagues (1957) Chu and Giles (1959) have studied
the effeata of rediation exposure inyitro on human tissue culture
preparations,
Only recently, hoverer, has it beeons possible to
undertake serial studies of chrowosone changes in the dircotly
irradiated human being.
Thie present cogummunication reports the
Presultea of sugh etwiies in tro patients given x-ray treatment for
ankylosing spondylitia.
the ehromesone preparations were made from blood cultures
useing en adaptation of the technique of Hungerford and his colleagues
(1959), the final epreading of the ochromesones being anehieved by
drying in air,
With thie technique a ceunt of the chromosomes
in 205 eelle from six meraal subjects showed 190 cells (92.68%) te
bave ¥S ehrenesence (fable 1). This proportion of sodal cells did
net @iffem eignificentig froma thet in o etudy of 489. cells from
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