Iron and Cobalt in Ecology which they were taken had been contaminated, The plankton contained only a small fraction of the total radioactivity, however, the amounts of radioactivity per unit volume, ine luding radioactive iron and cobalt, were much greater in plankton than water, In Table 3 the average percentage of total radioactivity contributed by the principal radionuclides in sea water and plankton at times less than 48 hours, one week, and six weeks after contamination are shown, At 48 hours the percentage compositions of water and plankton were Similar, indicating little or no selectivity. However, at one week cobalt-57,58,60, and jron-55,59 contributed 43 percent and 16 percent, respectively, of the total radioactivity in plankton and 0,05 percent each of the total ra- dioactivity in the water, Therefore, at one week the radioisotopes of iron and cobalt accounted for 59 percent of the radioactivity in the plankton but only 0.1 percent in the water. were At six weeks, radioactive tron and cobalt present in plankton at a level which ac- counted for 48 percent of the total radioactivity, each element contributing 24 percent of the total radioactivity. In the water, iron and cobalt accounted for 0,93 and 0.67 percent respectively of the total, The concentration factors exhibited by the plankton at six weeks after contamination were approximately 90,000 for iron and 100,000 for co- balt. The iron value is approximately one half of that reported by Krumholz and Foster (1957), for freshwater phytoplankton which have a much larger surface to volume ratio than do the zooplankton, The plankton considered primarily two trophic levels the phytoplankton or primary zooplankton. The zooplankton orous fishes (flying fishes) above comprise in the food chain, producers and the are eaten by omnivwhich are in turn eaten by the carnivorous fishes (tunas). The radionuclide composition of the two types of fishes have been discussed in another paper of this series (Lowman, 1962), and will not be conSidered in detail, however, the distribution pattern of cobalt-57,58,60, and iron-55,59, in the three trophic levels are as follows: Per cent of total Radioactivity Tine Total radioactivafter ity (disintregracontam-— tions per minute imation CobaltIronper gram of dry Plankton Onnivorous fish Muscle Liver Carnivorous fish Muscle ; Liver (weeks) 57,58,60 55,59 1 43 16 1 10 1 6 6 9 0.9 to 2,5 09.0 to 2.3 31 81 6 to 8 15 to 25 weight) 2.3 x 10° 22x 104 1.1 x 108 3.3 x to 1.2 x 3.6 x to 2.9 x 107 4 10, 10 5 10 On a dry weight basis the total radioactivity in the plankton was approximately two and 100 times that in the liver and muscle respectively of the omnivorous fish, The levels of radioactivity in the plankton and omnivorous fish cannot be comPared directly with those in the carnivorous fish (tuna) because the latter fishes are capable of "igrating great distance in short periods of time ond the length of time they had been in the area of Outamination before they were taken cannot be etermined, Page 565 _ If the per cent of total radioactivity contributed by cobalt-57,58,60, and iron-55,59 are compared in the plankton, the omnivorous fish, and the carnivorous fish, however, a pattern of accumulation may be seen. Whereas in the plankton radiocobalt contributed 43 per cent of the total radioactivity, in tissues of the omnivores it con=- tributed only nine to ten per cent and in the carnivores zero to 2.5 per cent. Radioiron accounted for 16 per cent of the total radioactivity in the plankton, 31 to 81 per cent in the omnivores, and six to 25 per cent in the carnivores. The reduction in the percentage of total radioactivity contributed by radioiron in the carnivorous fish was caused by a high concentration factor for zinc-65 in the tissues of these shes. Thus, the percentage of total radioactivity contributed by radiocobalt decreased through the two higher trophic levels, Radioactive iron, however, increased in percentage of total radioactivity in the muscle and liver of the omnivorous fish over that in plankton and was concentrated in the liver at an absolute level double that found in the plankton. In addition to the food chain represented by the plankton, omnivorous fishes, and carnivorous fishes, another food chain is comprised primarily of the plankton, omnivorous fishes, and sea birds, Among the birds at Eniwetok are the terns or sea swallows (fairytern, Gygis alba; common noddy tern, Anous stolidus) which feed primarily upon small fish and to a lesser degree upon squid. Analyses for iron-55,59 have not been made on the tissues of these animals,and in gamma Spectra of the liver, gut, kidney, muscle, and lung tissues of the terns, only the gamma peak of Zinc-65 was detected (Lowman, et al., 1957). Tron-55 is probably present in the tissues of the terns in large amounts, however, Indirect evidence has been found that this is the case in analyses of soil samples from areas in which these birds nest (Palumbo and Lowman, 1958). A comparison is shown below of soil samples taken in bird nesting areas and from an island where nests were not present, Per cent of Total Radioactivity in Soil Ce 144_p, 144 | Iron - 55,59 Cobalt - 57,58,60 Manganese - 54 Zinc - 65 Non-nesting area (Lowman, et al., 1957) 43.6 Nesting area (Palumbo and _Lowman, 1958) 41.7 0 40.6 1 to 2 0 0 0.4 0.8 0 In the non-nesting area the soil did not contain the radioisotopes of iron, manganese, or zinc, but did contain one to two per cent of radiocobalt. In the nesting area, zinc-65 was not found in the soil, which contained excretory products from birds although it was present in high amount in the tissues of the birds. Har~ ever, approximately 41 per cent of the total radioactivity in the soil was contributed by iron-55,59 with only trace amounts of radiocobalt and manganese, On the basis of the soils data and the gamma spectrum data on the birds, these animals excreted high amounts of iron-55,59, almost all of the cobalt-57,58,60, and manganese54, and retained the zinc-65, The fact that the gamma peaks of cobalt-57,58,60, and manganese-54 were not found in the bird tissues and that these radionuclides were present in low amounts in the soil, indicates that the birds ingested relatively low amounts of radioactive cobalt and manganese and large amounts of iron-55,59 and Zinc-65 and retained the radiozinc to a par A AVES

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