Wit
baa
ypataarh.
-
Adulitiomat
3 -
November 2},
1978
Peeth Samples Necessary:
a)
Coubtol
samples
for different categories,
b}
Pending the results trom the currently available samples,
additional samples for activity concentration determination
ane auboracdrography .
(to) i (a) +(e) & CE)
Le witli be obluined based un a flexible but carefully thought out
UXpurtmentabl desrvgn.
Cll
Methods:
Activily
Concentration
Each Marshall Ysland sample selected would be analyzed
for 238pu, 239,240py
Via the ion-exchange Lixalion-elution-electrodeposition technique and alpha
counting, For 945y vie the HEDIP extraction of 30y—sceparation as the oxalate
and beta counting, aml For 241aAm (if detectuble)
via the gamna counting of the
€luent.
Only the plutonium data will be used for F. ‘T. Cua's Ph.D. research.
Each animal tooth sample selected will be analyzed for 438pu or 239,240pya,
A
quality assurance proyram will be set up between BNL and the laboratory from
which the samples are obtained since the animal bone data are generated at the
Other laboratories.
Al] equipment is currently available at the Safety and Environmental Protection
Division.
Autoradiography
Numerous autoradiographic techniques for preparing histologic section of
undecalcified teeth are under evaluation.
Each human and animal teeth samples
selected will be subjected to the technique developed ultimately.
Each section
WiLL be kept for Future 20sr studies.
fNqudipment Necessary:
a}
b)
c¢)
qd)
e)
£)
g)
h)
veagents used in preparing the samples
bioplastic or paraffin wax
embedding apparatus
mechanical yrinder or microtome or microsawing apparatus
slides and emulsions and plates (a & B tracks)
photographic processing equipment
staining reagents
microscope (video camera???)
Question:
Would it be difficult to arrange time for the use of the equipment
currently available in SEPD and other departments such as Biology and
Medical?
Iv.
Results:
1.
238pu or 239,249 py activity concentration in the teeth samples of different
kinds of animals exposed through different routes of entry
2
Bone data
3
Yoeth
4.
(1)
to
Bune
(2)
ratios
for
different
animals
for
different
wobry
routes
.
of
2398p and 2492490 aclivity concentration of MI teeth samples as a funckion
olf
Ff
age,
sex,
extraction date,
2004956
kind of
tooth,
island,
etc.