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Natl. Inst. Radiol. Sci. Ann. Rept. (NRIS—10, 197 }
in the table, common clone cells in the two tissues
%
did exist in cases B-17 and Th-3.
if
3 4
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In case B-17, one of the fishermen exposed to
fallout radiation at Bikini in 1954, a clone with a
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and 70 of the 3,398 cells (2%) in the blood cultures
showed an identical karyotype of 46, XY, Gq-?,
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1945, 21 of the 519 cells (4%) in the bone marrow
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MINIMUM VALUES OF NEUTROPHILS
43
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In case Th-3, a man injected with Thorotrast in
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karyotype of 47, XY, mar+ occurred in the bone
marrow with a frequency of about 10%. In the
blood cultures four of the 690 cells (0.58%) showed
the karyotype of this clone.
Oo.
Fig. 10. Correlation between chromosome aberrations (Cs
cells)jand minimum values of neutrophils.
and the derivatives, but none of the 273 cells
snalyzed in the blood cultures showed this karyoty pe.
Common Clone Celfs with Structural Chromosome
Aberrations in Peripheral Lymphocytes and in Bone
Marrow of Irradiated Humans
Takaaki Ishihara, Sei-ichi Kohno
and Toshiyuki Kumatori
The Ph'
chromosome in chronic granulocytic
leukemia is known to be present in granulocytic,
erythrocytic and megakaryoeytic cells in bone mar-
row, but not in peripheral lymphocytes which are
stimulated to divide in culture responding to PHA.
The evidence has led to an idea that those lymphocytes might be developed from a separate line while
the former three cell series are derived from a common stem cell. In mice and rats, on the other hand,
the presence of multipotent lympho-hematopoietic
stem cells has been suggested.
As a means of approaching the problem on the
derivation of lymphocytes in their relation to bone
marrow cells in humans, three radiation-exposed in-
dividuals who showed presence of distinct clones
with structural chromosome abnormalities in bone
marrow were studied in order to find out whether
or not clone cells common to peripheral lymphocytes and bone marroware present.
The results of the chromosomeanalysis both in
the bone marrow and in the cultured lymphocytes
of the three cases are presented in Table 2. An seen
221
!
In case RT-1, a cervic cancer with hypoplastic
anemia received radio-therapy, nearly 100% of the
cells in the bone marrow were members of a single
clone showing a karyotype of 46, XX, U(Bp+;Cq—)
The identification of cells with the same radiation-induced markers among the dividing cells in
PHA-stimulated lymphocytes and in bone marrow in
cases B-17 and Th-3 seems to be conclusive evidence
for the presence of a lympho-hematopoietic stem
cell in man yielding both lymphoid and bone mar-
row descendants.
The present data trom irradiated humansclearly
demonstrated that PHA-responsive peripheral ly mphocytes are the progeny of the same stem cell for
bone marrow cells, but they do not explain the
reason for the absence in peripheral lymphocytes of
cells with the Ph' chromosome in chronic granulocytic leukemia or of the clone cells of case RT-1,
which are observed as the majority of the cells in
bone marrow. This is a serious problem to be solved
in future.
(Unpublished)