Table 6. MitoticallyConnected Abnormalities Cper 1,000 Erythroblasts) Subject 'Binucleated : No. | “T-3 + K-2° K— 4 K-35 K— 6 13 0.67 13 13 K— 8 K—9 K —10 K—1l K-12 K-13 K -16 Normal 33 0 2 1.3 4 0.67 2 27 | persons, occurred in them. Dicentrics were found Mitosis | in 7 cases, and monocentric abnormal chromo- 0.67 + somes were present in all of the cases examined. Also, aneuploid cells occurred in a high frequency. In view of the importance of these findings, long term observations are to be made. The chromosome abnormalities of these patients were reported elsewhere together with those of other radiation-exposed people, i.e. atomic bomb sur- . 11.3 | 2 | @ 6| og 1 “2 7.3 0 0 2 | 13 0 9g 3 0 0 0 067 0.67 — _ _ _ _ _ _ vivors, Thorotrast-injected patients, etc.'” ig | 1) _ _ G. Other Laboratory Studies Several laboratory studies were made on the subjects. The results of these examinations were mostly within normal range. However, some of them indicated slight abnormalities. ZTT: Normal. Thymol turbidity test: Cases K-5, 6, 8 and 9 revealed high val- | 0 _ ' 9 | 1 | | | 4 43 0.67 0 O 1.3 _ — + _ _ _ | 1 2 2 4 4 0 3 0 0 5 2 0 4 Giant jRaryomerc! come in Neutrophils 4 T—8 K—-1!' \ Cheomen Erbl. 0 | 0 dicentric chromosomes and monocentric abnormal chromosomes, chromosome-type abnormalities which have never been observed in normal | o _ _ ues (7~ 10). Icteric index: Cases K-8, 11, 13 and 16 showed slight increase (nearly 9). Serum alkaline phosphatase : Cases K-6, 7, 10 and I] In the histological sections, all showed normal cellular marrow. c. Haemorrhagic diathesis As to the haemorrhagic diathesis, no abnormalities were found. F. Chromosome Studies Chromosome studies succeeded on 13 of the exposed persons.The observation of chromo- somes was made by cultures of peripheral leucocytes prepared in accordance with the method by Moorhead, ef al. with a slight modification. Samples were obtained 72 hours after initiation of culture, and 200 metaphases were examined in each culture for all the cases except one case. The control observations were based on 1] normal males, aged 28-53. in- unit). GOT: No pathological data was obtained except for T-7 (70 Frankel unit). GPT: AI! data were within normal range. Paper electro phoresis: No abnormal findings were obtained. Total cholesterol: All were normal. BSP test: Cases T~6 (12.593), K-6 (5-7.5%) and K-13 (5-7.5%) revealed slight high retention (45 min.). Erythrocytes sedimentation rate: All were normal. Tests for syphylis: All were negative. Routine urine analyses: A sample from Case T-7 was positive The results of the observations are shown in Table 7. As shown in the table, frequencies of chromosome aberrations in the exposed persons were much higher than those in the normal. Further, indicated creased values (12~18 King-Armstrong for sugar (1/10~1/4%). Others were normal. feces: No parasites were observed. PBI: Five cases were measured for PBI con- tent by courtesy of Dr. Tachibana (St.