AQLEID ,
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4
M. ANDERSEN
Murdsugh, H. V. Jr. Schmidt-Nielson, B., Doyle,
E. M. & O'Dell, R.: Renat tubulac regulation
of «wea excretion in man. J. appl, Physsol. 13,
263, 1958.
;
Murray, J, Merni, J. and Hartwell Harrison, J.:
Kedney transplantation between seven pairs of
édeinical wan. Anm Surg. 144, 143, 1958.
Rhoads, C. P., Van Styke, D. D, Heller, A. &
Almg, A. S.: The effec of novocainisation
and total section of the nerves of the renal
pedicle on renal blood flow and funcomnm Amer.
J. Physiol. 110, 02, 19h.
Sartorans, O. W. & Burlington, H.: Acute effects
af doncrvation on kidney function in the dog.
Ibid 185, 407, 1956.
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gcandinat Jd. Cham. & biahe duc stry atin oes
fe
Schmadt-Nielsen, H.: Urea excretion an white lay
and kangaroo rats a inthucuced by EXCiLeen
aral by dict. Ited 18/, 131, 1955.
Study, K. S. & Shipley. R. E.: Effect of tenad
antery-nerve stamulation on renal blood The
measured derecthy and by olsarance and
ton of déiodrast. Ibid 159, 92, 1949.
Teusta, J., Barckey, A. E., Daneel, O. M., Franklin,
K. J. & Prechard, M. M.: Studios of the reny
corculation Oxford 1948.
Quinby, W. C.: The funceton of the kidney wher,
deprived of ite norves J. exp. Med 23, 535
1916.
,
Youk, E., Kerr, W., Wilson, D. and Jaworski, Z.
The diagaosis aml treatment of remad hyper.
tenon Amer. J. Med. 28, 169, 1960.
EXPERIENCES WITH THE RADIOCHROMIUM METHOD
FOR DETERMINATION OF RED CELL VOLUME!
By R. WENNESLAND, E DROWN, J. HOPPER, JR, K. G SCOTT, J. L. HODGES, JR.
any BL AKADLEY
From the Uepariment of Medicine, the Cardiovascular Research Institute,
the Rodtoachwity Research Céenter,and the Department of Statistics, University of Califoruss,
San Francisca and Berkeley, Cabfernus
(Recewed for publication Decanber 18, 1461)
The use of radioactively lalicled red cells
jor the determination of the red cell volume
(Vebc) has gained wide acceptance since its
introduction by Hahn & Hlevesy (1940) Of
the procedures currently in use, the method
IMood is not crifical, as is the case with P3?
(Sterling & Gray 1950). In subjects without
hemolytic disorders, the apparent volume of
distribution of cells kubeled with Crél even
24 hours alter injection ig no more than
5 per cent greater than the volume estimated
of Sterling & Gray (1950), utilizing auto-
within an hour of the injection (Sterling &
genous cells labeled with radivactive sodium
chrpmate(Cx**), meets most adequately the
basic requirements for bloud volume meas-
Gray 1950; Nomaof, Hopper, Brown, Scott
& Wennesland
1955S).
urement by the dilution principle: a) tlt
| the “indicator”, in this case tayzged cells, can
1954,
Mollison
&
Veuall
One of the principal disadvantages of
I become evenly distributed in the entire blood
wing either Cro! or 2? is that the cells must
he tagged in wiiro, which in clinical studies
means that the subject must be available at
volume within a reasonable period, and /)
that none of the tag is lost from the circulat, Ing blood during mixing. Vhe rate of loss of
Ce from the tagged cells is so slow that
the lapse in time between their injection ai
the estimation of their dilations the subgect's
least an hour before the actual measurement.
More mipottint, however, is the possible
ctheet on accuracy of damaging the cells by
processing: them ae titre. As will be shown,
this is not an important source of error ia
volume delermaations (Wennesland, Shepherd, Nomof, Hiown, Hopper & Bradley
1957), except an patients with hemolytic
Iendencies. Greater care in tagging is) required if the cells are to be used for studies
‘Thies work was supported hy grants (E1-1271
j ad RG-3666) from the United States Public
Health Service and by funds from the American
Heant Assoctation, the Moiterey ate! San Fram ince
eave Associations aul the Cosmunttec on Reseach
Ot the University of California School af Meds tie
Wi
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