Besides the usual thorough clinical work-up of the patient inclading: hematologic studies, estimation of the character and weight of the[ thyroid, systematic recording of all features of ophthalmopathy, the PBI, tke uptake , of 2317 and the clinical judgement of the severity of the hype pidiem, a series of special observations were made on these selected patiesgts. is these special studies that have been supported by this grant. ey inelude the following: 1) The uptake of the treatment dose by the and repeated (almost daily) observations thereafter to determine of disappearance of the 1512 from the thyroid over a period of up It thyroid te pattern fo three weeks. 2) Sampling of the total radioactivity per ml of blood ovar the frame period of time. 3) Serial quantitative chromatogrems to show the cmounts of various iodinated compounds in the blood (5 to 8 sampleg per patient) to reflect the changing pattern of these compounds following the administration of the treatment dose. 4%) Similar observations on jthe urine with attention to the daily total loss of radioactivity from/the body. These serial observations not only initially reflect the abnormalities of the disease process and variations among patients before a substantial radiation effect occurs, but they aleo reflect changes that are ind d the radiation. The data have been gradually accumulated and subsequent evaluation of the course of the disease has been considered in the Right of these findings. The cases represent a wide range of response. ‘The ultimate value of the data is not fully know because the long term effects of 1312 are still coming to light. Our laboratory repres2nts one of 19 centers included in the Radibiodine Therapy Follow-Up Study of the Radiological Division of the United Btates Public Health Service. (We received a small grant from this source solely for the purpose of tracing and keeping contact with all of these special patients as well as others treated in ow center without special stpdy). The detailed data described above on approximately 18S of these patients, etudied under this Atomic Energy Contract, have proven to be the mo thorough in this national follow-up study. The kinetics of the 131f in these treatment patients, along with the collateral observations, se 131r. : now as tie basis for attempting to define the patterns of behavior of A special committee headed by Dr. Mones Berman has been set tp (May 1967) to study this material. It is hoped that after the committee/has established kinetic patterns from these detailed data that fragnents of data from other less completely studied patients can be analyzed anq the missing data estimated for those patients by the use of computers. [The reasons for the variations in the therapeutic ocutcome may thus be l¢arned in a large mumber of patients now being followed. Additional and mre meaningful information should come from the data assembled under thq Atomic Energy Commission contract as we follow the outcome of the radiatic} in the patients and have the collaboration of others who are more knowledgeable in the study of kmetics and who are using our data. We continue to carry out a detailed study on selected patients when: 1) an appropriate patient is to be treated, 2) when he is available for intensive stay, and 3) when the personnel working under the contract have a suffidient block of consecutive days to complete the study of blood and urine af that patient. DNA Synthesis in Radiated and Stimulater .: Thyroids Gradually, as the project has progressed, emphasis has shifted somewhat more from the purely physiologic toward the morphologic changes caused by