10 Table 6. Mitotically Connected Abnormalities dicentric chromosomes and monocentric abnormal (per 1,000 Erythroblasts) chromosomes, . | Aberrant Subject Binucleated Karvomere ChromoGiant No Erbl. ; some in Neutrophils us ' Mitosis T— 3 T- 8 4 4+ K-— 2 K-- + KR 5 13 0.07 1.3 K— 6 K— 8 K-— 9 13 0 2 K-11 + K -12 K-16 K— | K -- 10 3.3 in 7 cases, and monocentric abnormal chromosomes were present in all of the cases examined. 7.3 2 — chromosome abnormalities of these patients were 5 0 1.3 0 — _ reported elsewhere together with those of other radiation-exposed people, i.e. atomic bomb sur- 0 + —_ — vivors, Thorotrast-injected patients, etc.'” he 2 11.3 0 | YU — 0 - 27 0,67 L.3 — 1 2 + 2 2 £ 0 0 _— 3 0 0 0 _ 2 0 0 067) 43 _— _ 0 _ 0 ! Also, aneuploid cells occurred in a high frequency. In view of the importance of these findings, Other Laboratory Studies Several laboratory studies were made on the subjects. Vhe results of these examinations were mostly within normal range. However, some of them indicated slight abnormalities. ZTT: Normal. Thymol turbidity lest: Cases K-5, 6, 8 and 9 revealed high values (7~10). Icteric index: Cases K-8, ll, 13 and 16 show- ed slight increase (nearly 9). Serumalkaline phosphatase: Chromosome Studies Chromosome studies succeeded on [3 of the exposed persons. The observation of chromosomes was made by cultures of peripheral leucocytes prepared in accordance with the method by Moorhead, et al. with a slight modification. Samples were obtained 72 hours after initiation of culture, and 200 metaphases were examined for all the cases except one case. The control observations were based on i1 normal males, aged 28-53. unit). GOT: No pathological data was obtained except for T-7 (70 Frankel unit). GPT: Alt data were within normal range. Paper electro phoresis: No abnormal findings were obtained. Total cholesterol: All were normal. BSP test: Cases T-6 (12.599), K-6 (3-7.593) and K-13 (5-7.5%) revealed slight high retention (45 min.). Erythrocytes sedimentation rate: AH were normal. Tests for syphylis: All were negative. Routine urine analyses: A sample from Case T-7 was positive The results of the for sugar (1/L0~1/429). Others were normal. observations are shown in Table 7. As shown in the table, frequencies of chromo- sume aberrations in the exposed persous were much higher than those tn the normal. indicated in- creased values (12~18 King-Armstrong In the histological sections, all showed nor- mal cellular marrow. c. Haemorrhagic diathesis As to the haemorrhagic diathesis, no abnormalities were found. The G. Cases K-6, 7, 10 and 11 in each culture normal long term observations are to be made, _ Nororal F. in persons, occurred in them. Dicentrics were found _ 0 0.b7 3 abnormalities observed 0 i 2 0.67 4 been 2 | v chromosome-type never + — | 2 have 0.67 0 o K-13 13 3! 0 which Further, Feces: No parasites were observed. PBI: Five cases were measured for PBI con- tent by courtesy of Dr. Tachibana (St.