Nuclear Medicine Technology and Other Health Applications Project Title: Metabolism of Carbon Labeled Compounds RX-01-03- (e) 14, Scope: (Cont'd) metabolites was first studied using the same body weight and dose levels that would be used in humans, and has now been extended to humans. results obtained with the isotopically labeled compounds should help determine the specific abnormalities in the tryptophan metabolism in The patients and eventually produce information that may lead to better therapy. Patients with scleroderma, siderosis, pellagra, and hepatic tumors are studied in South Africa. Malaria is studied in Stateville Prison, Illinois, and in South Africa. The labeled compound isolation and ana- lytical work is done at Brookhaven, though in the past, was accomplished at the University of Wisconsin. 15. some of this work Relationship to Other Projects: (Hankes) . Studies related to the work on carbohydrate and lipid metabolism include those at Brookhaven of Steele, Department of Biology; Dahl, Medical Research Center (see RX-01-03-b); Atkins (see RX-01-03-c); Wolf and Christman, Department of Chemistry; and Newman, Department of Applied Science. At Los Alamos Scientific Laboratory, related work includes the collaborative studies with Ott and Gregg on production of C-13 labeled organic compounds for metabolic studies; MacInteer on analyses of 13¢9, ; and Matwiyoff on NMR analyses of glucose-C-13. Klein of Argonne National Laboratories collaborates on analysis of C-13 labeled compounds. Related studies ac other institutions include those of Waterhouse, University of Rochester; Searle, V.A. Hospital, San Franciso; Kreisberg, University of Alabama; Pollycove, San Francisco General Hospital, University of California; Da Costa, Radiation Medicine Centre, Bombay, India; Segal, Pennsylvania Medical College; Shoop, University of New Mexico; Hetenyi, University of Toronto; Kalant, University of Montreal; Long, Columbia University; Farquhar, Stanford University; Bierman, University of Washington; Nikkila, University of Helsinki; Sailer, Universitatsklinik in Innsbruck; MacDonald, Guy's Hospital Medical School, London; Nestel, Australian National University, Canberra; Chytil, Vanderbilt University and Curzon, Institute of Neurology, London. (Shreeve) erally, tryptophan metabolites Labeled with carbon 14 in positions @riginate from specific positions of the benzene ring or the mucleus are unavailable due to difficulties encountered in their synthesis, and separation of their D- and L-isomers in satisfactory yields. Although studies with carbon-14 labeled isomers of this type of compound are conducted only at Brookhaven, studies in other laboratories include those of: Altman, N.Y. Medical College, on hormone effects on tryptophan pyrrolase enzyme; Gholson, Oklahoma University, on feedback control mechanisms; Lardy, University of Wisconsin, on (See Continuation Sheet) [119240 RX-82 '