Nuclear Medicine Technology and Other Health Applications Project Title: 14, Seope: Lymphocytopoiesis and Transplantation Immunology (Cont'd,) RX-01-03-(d) When the human renal transplant program commences at Nassau County Medical Center, patients will be randomized into ECIB prepared and nonECLB prepared groups for evaluation of ECIB as a pre-transplant immunoSuppressive procedure, Evaluation will require at least 100 patients in both groups, With the estimated renal transplantation rate of 40-50 per year it will take about 5 years to evaluate ECIB. The toxicity of B, pertussis precludes its clinical use, It is necessary to resume work on this lymphocytosis-producing agent in order to separate non-toxic lymphocytosis-promoting factor, Another question that remains unanswered is the role of stress-induced secretion of adrenal cortical steroids on induction of lymphocytopenia during ECIB and ECIL., This problem will be studied by performing ECIB and ECIL in normal, -wdrenal- ectomized, and sham-adrenalectomized calves, . The culture of cells in intraperitoneal diffusion chambers described in RX-03-02-(b) has provided provocative data on lymphocycic growth, Human blood cells in diffusion chamber cultures show a sequence of events Suggesting that small lymphocytes are undergoing transformation and proliferation into lymphocytoid blasts with production of plasma cells after 2-3 weeks in culture, The diffusion chamber system will be applied in the study of immunological reactions. 15, Relationship to Other Projects:. Closely related studies at Brookhaven are the studies of hemopoiesis (RX-03-02-b) and Stoner's studies on lymph nodes during antigenic scimulus (RX-03-Ol-b), At Oak Rid Makinodan studies cellular aspects of humoral antibody production durin. aging, and Hanna studies immunological systems in relation to cancer, with emphasis on cellular immunity, Cottier, University of Bern; Jansen, South African Atomic Energy Board, Pretoria; Killmann, Copenhagen; Fliedner, University of Ulm; and Schiffer, Allegheny General Hospital, Pittsburgh, all alumni of Brookhaven, continue to work in closely allied areas and in collaboration with BNL, Related studies elsewhere include those of: Thomas and associates, University of Washington, School of Medicine, on ECIB in leukemia and bone row transplantation; and Hollard and associates at Centre d'Etudes 'g de Grenoble, France, on ECIB and its application in the treatment ia. McGinn and associates af, the University of Cambridge, Persson ciates at the University of Goteberg, and Weeke and associatcs, Rigshospicalet, Copenhagen, apply ECIB in the preparation of patients for (See Continuation Sheet) 1179228 RX-70