Nuclear Medicine Technology and Other Health Applications Interrelationship between Genetic and Environmental Factors Project Title: in Clinical and Experimental Hypertension RX-01-03-(b) 16. Technical Progress in FY 1973: (Cont'd.) “normal” insulin levels contrasted with 26, 18, and 56% in the controls. After correction for age, weight, and sex, the differences were not significant. It is concluded that there is a signficant association between hypertension and reduced glucose tolerance but probably not an increased insulin response to a glucose challenge in these same patients. A recent study of the 24-hour excretion of uric acid in patients on a constant low-purine diet indicates that hypertensives have an increased excretion of uric acid not explicable on changes in renal uric acid clearance but probably reflecting a larger-than-normal "pool" of uric acid. 17. Expected Results in FY 1974: Kidney transplant studies will continue. . Injection of antikidney serum (AKS) will be repeated to see if AKS nephritis can be provoked in R rats. Also the experimental pyelonephritis study will be repeated as soon as Dr. Pavlova can help with the bacteriology. The initial adrenal transplant study will be completed. Study of renin activities in plasma of rats with renal homo- and auto- grafts will be expanded. The preliminary exploration of the usefulness of operant-conditioning as a technique for inducing hypertension should be completed allowing decision as to feasibility of expanding or discontinuing the study, initiated, A new study of uric acid pools in human hypertension will be Exploration of the renin-angiotensin system will continue, in particular with reference to the factor that modified the in vivo response to angio- tensin. The study of effect of NaCl on Japanese “spontaneously hypertensive rats'' and the study of influence of gonadectomy on BP should be completed. Arrangements have been completed to begin kidney micropuncture studies some time during the next year in the laboratory of Dr. Alain Grandchamp of the Department of Medicine at the University of Geneva Medical School. Such studies may be critical in understanding the recent finding that sodium is handled differently by the kidneys of the two strains and may provide fundamental insight into the pathogenesis of hypertension, 18, ted Results in FY 1975: Ss 19, es will continue as dictated by findings in the previous fiscal year, Description and Explanation of Major Materials, Equipment, and Subcontract Items: None (See Continuation Sheet) L1V92i1 RX-53