Biological Studies of Materials Arising from Energy Operations The Effects of Pollutants on Cells Studied in Project Title: Tissue Cultures 15. Relationship to Other Projects: BX-03-06-(c) Related studies on pulmonary function, pulmonary surfactants, and elasticity are proposed by the BNL Department of Applied Science, The experiments would lean heavily upon existing tissue culture techniques for the study of hemopoietic stem cell proliferation and differentiation and studies on renal glomerular cultures and studies on the induction of dominant lethals by tritium (RX-03-02-b and RX-01-Ol-c). Dr, Aaron Janoff, State University of New York at Stony Brook, is actively pursuing studies aimed at confirming his hypothesis on the release of Lysosomal enzymes from neutrophils and macrophages as an etiologic factor in the production of emphysema and other chronic pulmonary disease, Cotemporary studies on @] antitrypsin are closely related since individuals with congenitally determined pulmonary disease have an absence of or marked reduction in a) antitrypsin, 16, Technical Progress in FY 1973: Project not yet initiated, 17. Expected Results in FY 1974: Project to be initiated in FY 1975. 18, Expected Results in FY 1975: Design studies will center on the development of environmental incubators for tissue culture techniques and preliminary studies will be made on producing plaques in the three layer tissue culture proposal for detection of pollutants harmful to the lung, With the appointment of additional scientists and technicians, tissue cultures will be established and control studies initiated to see the effect of gaseous pollutants (oxides of nitrogen and sulfur) upon the proliferation rates and the development of cytogenetic abnormalities. Incubators within which one can control the concentration of ambient gases will be designed and fabricated. Tissue culture flasks and dishes will be modified in order to insure a constancy of the concentration of the gases overlying the culture, The three tier culture technique to ticulate pollutants will be established, When concentration of mful to tissue culture are established and hazardous particulates Hified in the tissue culture system, facilities for long-term animal f will be designed and fabricated. (See Continuation Sheet) 1119335 RX-392