Biological Studies of Materials Arising from Energy Operations The Effects of Pollutants on Cells Studied in Project Title: Tissue Cultures RX-03-06-(c) 13. Publications: Project not yet initiated, 14, Scope: A) 200 Word Summary: The incidence of chronic pulmonary disease is increasing, Pollutants in the environment, cigarettes, and industrial dusts are suspected as the primary causes, Evaluation of suspected hazards by exposure of animals in closed, monitored environments would take decades and be extremely costly in manpower as well as dollars. Using a 3 tier tissue culture system, it is proposed to study the interactions of pollutants with pulmonary macrophages and neutrophils and to detect those pollutants with deleterious effects on human pulmonary epithelium. In addition, the effects of noxious particulate and gaseous pollutants will be studied in classical tissue culture systems, The pollutants shown to have deleterious effects in culture will be evaluated in animals, B) Supplement to 200 Word Summary: In the highly industrialized western society, man is subject to a changing incidence of genetic, cardiovascular, pulmonary, neurological and mental diseases and cancer. The incidence of pulmonary diseases is increasing but the suspected role of the environment in causing this increase has been inadequate- ly evaluated, One must consider factors: the effect of radiation nuclear power plants, industrial foods in the diet; the effect of effects of chemicals used the following as potentially causative from all sources (the practice of medicine, operations, etc,); the effect of processed drugs used in the practice of medicine; the in industry; effects of industrial gas and liquid effluents contaminated by heavy metals; the carcinogenic effect of hydrocarbons; the general toxic effect of oxides of nitrogen and sulfur exhausted from fossil fuel plants; the effect of inner-city crowding on the spread of disease generally; and the effect of stress due to such influences on the individual as the uncertainties connected with modern national and internationa competition, None of these factors can besatisfactorily considered separately Each must be analyzed in relation to the others, the varying interactions thoroughly evaluated, Studies comparable to those used to assess the hazard of inhaled B and uranium are required to identify the chemical and physical char@gistics of hazardous pollutants, the long-term animal studies will 1-2 decades, A method enabling more rapid answers would be useful in designing the long-term experiments. Thus, the initial proposal is to study the effect of environmental contaminants upon cell proliferation and function, including in vitro carcinogenesis, with diverse standard tissue culture systems, These would include in vitro cultivation of bone marrow (See Continuation Sheet) 1119333 RX - 390