Project Title: 13, Molecular and Cellular Radiobiology Storage and Transfer of the Genetic Message RX-03-02-(d) Publications: Kim, C. H., Jamar, M. P., and Hamilton, L. D. delayed hypersensitivity to Trichinella spirilis: J. Exp. Parasitol. (in press). Mechanism of transfer of the effect of immune serun, //3¥ Jamuar, M. P,, Kim, C. W., and Hamilton, L. D. Fine structure study of lymphocytes from animals hypersensitized to Trichinella spirilis antigen, J, Exp. Parasitol. (in press), JIL McVicar, J. We, Richmond, J. Y.; Campbell, C. H., and Hamilton, L. D. Observations of cattle, goats and pigs after administration of synthetic interferon inducers and subsequent exposure to foot-and-mouth disease virus, Canadian J, Comparative Med. (in press). 4 //300 14. Scope: A) 200 Word Summary: The long-range objectives of this program remain: understanding how radiation and other genetic chemical hazards in the environment cause human disease, especially cancer, Efforts are aimed at establishing the various nucleic acid structures and their interaction among themselves and with environmental chemicals with the greatest possible accuracy, Approaches include: study of the 3-dimensional structure of natural double-stranded DNA, RNAs and of synthetic polynucleotides and of their complexes with other ‘ molecules such as histones and novel chemicals; study of the role of RNAdependent DNA polymerase in proliferating normal, tumor, and immunologically stimulated cells; and study of the effects of synthetic polynucleotides : on viruses, If synthetic and biological double-stranded RNA's continue to be superior as inducers of interferon and immune responses in general, and at such dosage as to be therapeutically practical, they will merit testing against a wide variety of viral diseases in animals and man, and as promoters of the immune response after radiation injury. While it is clear that in the first instance such tests might be confined to established viral diseases, in the event that the synthetic and biological polynucleotides prove to have no harmful side effects, they would warrant trial in disease in man where virus etiology is suspected though not proven, This might offer possibilities treatment of several malignant diseases, Additional effort is needed flep nucleoside analogs for polynucleotide synthesis and to prepare ate biological polynucleotide complexes; ' potent, B) such polynucleotides may Supplement to 200 Word Summary: Since the only way in which environmental hazards--radiation and chemical pollutants--at low doses and low dose rates could be accurately assessed would be directly from a precise understanding of how they induce (See Continuation Sheet) 1179309 RK-249