Molecular and Cellular Radiobiology Project Title: Mechanisms of Action of Free Radicals and Hormones 13. Publications: Le Fevre, M. turtle bladder. E., Dox, L. J., and Brodsky, W. A. of peptide chains. Borg, D. Metabolism of depleted J. Membrane Biol. 8, 205-18 (1972). Glass, J. D., Schwartz, I. L., and Walter, R. solid supports through the dinitrophenylene moiety: RX-03-02-(c) Jie’ 7 / Bridging of peptides to bidirectional extension. J, Am. Chem. Soc. 94, 6209-11 (1972). C., Fajer, J., Forman, A., Felton, F., . + Vic hk 4C and Dolphin, D. Pi- radical ions from the oxidation and reduction of chlorins, chlorophyll and bacteriochlorophyll. Presented at IVth International Biophysics Congress, Moscow, August 1972, / oF Glass, J. D., Walter, R., and Schwartz, I. L. Bidirectional ptptide synthesis on a solid support. Presented at the 12th European Peptide Symposium, Halle, East Germany, September 1972. / 7699 Le Fevre, M. E., Dox, L. J., Mc Clain, O. M., and Brodsky, W. A. of tissue swelling by alteration of orientation of tissue sacs. Comp. Physiol. 41A, 319-25 (1972). Le Fevre, M. E, —_——__ Le Fevre, M. 14, in the E., turtle /L/GS Effects of ouabain and high K+ on respiration of turtle brain and urinary bladder in vitro. cells Induction Biochem. _ Comp. Biochem. Physiol. Reincke, U., Arbas, R., and Gennaro, bladder, Anatom, Rec, ( in press ) J. _ (in Press)1 23034 a F. Lymphoid / /. Y EC Scope: A) 200 Word Summary: Free radicals are involved in a number of fundamental biological reactions, especially those of oxidoreduction. Radiobiological effects, including sensitization and protection, depend largely upon competitive free radical reactions. Free radicals are implicated in some of the interactions of pollutants and carcinogens with tissue constituents, Many reactions of oxidative metabolism involve free radical pathways, as do the properties of some hormones and drugs. This program investigates free radicals as reactive intermediates in normal and pathological metabolism by seeking to identify and characterize free radicals found in tissue and biological substances. Present projects study radia } and radiation-like chemical reactions of biomolecules and correlate thes wh reactions of pollutants and carcinogens. Magnetic resonance py (EPR) provides foundations for most experimental work on free radicals. The Laboratory has proficiency in studying tissues and short-lived biochemical free radicals by EPR, having pioneered on-line computer applications to this research. (See Continuation Sheet) 11192496 RX- 236