Molecular and Cellular Radiobiology
Effects of Radiation and Chemicals on Control of
Hemopoiesis
RX-03-02-(b)
Project Title:
13.
Publications:
Sordat, M., Sordat, F., Cottier, H., Hess, M. W,, Riedwyl, H., Chanana,
and Cronkite, E. P.
Studies on lymphocytes. XV, Analysis of the in vivo
division cycle of large lymphoid cells in calf thoracic duct usingcombined
microspectropnotometry and autoradiography.
Exptl. Cell. Res. 70, 145-53
(1972).
.
JED
Nieto Garcia, M., and Johnson,
H.
A.
Cell Tissue Kinet. 5, 331-9
(1972).
Johnson,
H.
A.,
and Pavelec,
spontaneous less of cell function.
Cronkite,
E.
P,
M.
Ale Ul» l:
Thermal noise
in cells.
A cause of
Am. J. Pathol. 69, No.1, 119-29 (1972), (
Kinetics of granulopoiesis.
s
foes /
3
Presented at the Gesellschar
Fur Nuclearmedizin
u
zin E.D., Freib
reiburg, Germany, S September, 1972.
1972
FIV 3
/ JANA
Chanana, A. D., Schaedeli, J., Hess, M. W., and Cottier, H.
Predominance
of theta-positive lymphocytes in gut-associated and peripheral lymphoid
tissues of newborn mice.
J. Immunol. 110, No. 1, 283-5 (1973).
J O98
14,
Scope:
A)
200 Word Summarv:
The broad objective in this research is an understanding of the
injurious effects of radiation, drugs and chemicals on hemopoiesis, and
the processes of recovery from these effects.
Knowledge from these
studies should improve understanding of diseases of hemopoietic cell
differentiation such as leukemia, hypoplastic anemia,
lymphoma, myelofibrosis,
polycythemia vera, and thrombopenic and granulopotetic states.
Description
of the cell-to-cell interactions within bone marrow, definition of the
feedback loops controlling differentiation into cell lines, and a
quantitative measurement of the kinetics of cell proliferation is attempted
in order to define the chemical basis of regulation of new blood cell
These mechanisms must be determined in order to define how the
production,
genes in the hemopoietic stem cell (HSC) are switched on and off to
maintain the size of the stem cell pool, replete it and control differentia-
‘tion and amplification upon demand for blood cell production.
A battery of
techniques are adapted or developed for studies of hemopoiesis following
Legpetacics or administration of drugs and chemicals to animals, and
n fant and diseased states of animals and human beings.
B)
in
Supplement co 200 Word Summary:
hemopoietic
Among the techniques utilized in these studies are:
blood cell
and
cell growth in diffusion chambers; in vitro bone marrow
culture;
splenic colony growth
from the HSC;
cell kinetics using tritiated
(See Continuation Sheet)
[119285
1
Cell proliferation kinetics in
goldfish acclimated to various temperatures.
'
A.
RX-225