Project Title:
17.
Effeccs of Radiation on Living Organisms
Radiosensitivity of Immune Responses and Mechanisms of
Immune Reactions
Expected Results in FY 1974:
RX-03-0)-(»)
(Cont'd.)
Previous experiments on enhanced responses have been done with either
xenogeneic or allogeneic antisera complexed to BSA or FTT.
It seems likely
that there may be at least two components which account for the enhanced
response; one which is effected through the foreign antigenic determinants
present on the gamma globulin and one which is effected by the antigen-antibody
complex per se.
To eliminate the possibility that the enhancement is due to
foreign antigenic determinants, studies on enhancement will be performed with
Lsogeneic antisera complexed to the antigen thus studying influence of the
complex without possible xenogeneic or allogeneic influences.
A series of experiments will test the hematological and immunological
side effects of treating mice with TAP and CAP.
These experiments will
deal with the immediate toxic effect, comparative dose effects and duration
of daily treatment in relation to immonusuppressive effects,
Several studies
with Cottier et al. on the incorporation of tritiated-histidine into lymphoid
cells and specific antibody will continue.
(Stoner)
The initial phase of the project on tumor virus infection and tumorgenesis will deal with in vitro studies on the effects of radiation on avian
and mammalian cell cultures infected with three tumor viruses:
RIF virus
(RNA - Type I), T virus (RNA - Type III), and JM virus(DNA - Type II).
Fertile eggs from genetically susceptible and resistant chickens to leukosis :
will be utilized for the preparation of chicken embryo fibroblasts.
(Pavlova)
Fd
18.
Expected Results in FY 1975:
Acquisition of information on the genetic control of antibody responses
will allow selection of inbred strains and new recombinant
inbred strains for
use in study of lymphoid cellular kinetics during antibody responses.
anaphylactogenic properties of antigen-antibody complexes prepared from
The
specific immunoglobulins and enzymatic digest fragments of antibody will be
tested in irradiated mice and guinea pigs.
Studies will continue concerning
the efficacy of immunization of laboratory animals and man with complexes
prepared from specific human immunoglobulins,
Increased emphasis will be giver
to poor antigens and particulate antigens.
The study of hematologic and
immunosuppressive side effects of thiamphenicol and chloramphenicol will be
completed.
(Stoner)
in vitro studies will be continued and augmented by in vivo
nts to determine the effects of various doses of radiation on chickens
ally susceptible and resistant to leukosis.
viruses will be used:
RIF virus
and JM virus (DNA - Type II).
(See Continuation Sheet)
b4b492 18
The following tumor
(RNA - Type I), T virus (RNA - Type IIT)
(Pavlova)
RX-181