37
counts and types, alkaline phosphatase staining,

and basophil counts-e£4000 white cells showed no

evidence of leukemia or leukemic tendency. One
childin the irradiated group had 3% basophils but

no other positive findings. The cardiovascular and

arthritis surveys, as well as the general results of the

physicalexaminations, have not shown any apparent increased incidence of degenerative diseases
in the eXposed people. No radiation-induced
cataracts have been observed in anyof the exposed
people.

Genetic effects have not been specifically studied
because of the small number of people involved.
No apparentradiation-induced genetic changes

have been detected on routine physical examina-

tion in the first-generation children of exposed
parents.
Hematological surveys again showed considerable
fluctuation in the year-to-year meanlevel of leukocytes in both the exposed and unexposed groups.
The mean leukocyte level of the exposed group

showed a marked decrease at the ume of the 1960
survey (no unexposed people were examined). The
reasons for these fluctuations are not apparent. At
5 years post exposure, exposed people still had
mean platelet levels 10 to 15% below those of the
unexposed group. However, lymphocyte levels ap-

peared for the first time to equal those of the unexposed group. Mean erythrocyte levels were also

slightly lower in the exposed people. These blood

elements in the Ailingnae group also showed some

slight depression below the unexposed levels but

not quite so marked as seen in the Rongelap ex-

posed group. A general anemic tendency was

noted in all the Marshallese, both exposed and

unexposed. Price-Jones curves, on the average,
showed a slight microcytic tendency. Serum tron

levels were generally normal. The fact that some
of the blood elements in the exposed group have
not yet returned to the levels in the unexposed
group raises the possibility that a residual radiation effect on the bone marrow persists, but other,

not immediately apparent, factors may be involved.
.

Studies ofgenetically inherited characteristics. Blood
grouping studies in the Marshallese showeda rela-

tively high B gene frequency, a high N genefre-

quency, an extremely high R' gene frequency, and

total absence of Kell and Diego factors. These
characteristics differ from those of Polynesians and
suggest relationship with Southeast Asians and
Indonesians. Haptoglobin studies showed the fre-

quency of the Hp‘ gene to be higher than in

European populations thus far tested and consistent with populations living near the equator.
The distribution of haptoglobin types showed the
population to be relatively homogeneous. Trans-

jerrins in all sera were type CC, the common Euro-

pean type. B-dA mino-iso-butyrie acid urinary levels
showed the Marshallese to be the highest excre-

tors of this acid of anv population thus far re-

ported. Levels in the exposed group were about
the same as in the unexposed group, and no cor-

relation was found with body burden level of
radionuclides; this indicates that there is probably
no correlation with radiation exposure. Glucose-6-

phosphate dehydrogenase of the red cells appeared to
be deficient in the Marshallese. Considerable caution must be exercised in evaluatingthe results of
these studies on genetically inherited character-

istics because of the small number of samples
tested. The data do seem to indicate relative
homogeity of the population and closest kinship

with people of Southeast Asia. These data also
may be useful as a base line should genetic changes”
appear in later generations, possibly related to.
radiation exposure.

:

Results of other laboratory studies carried out

during the 5-year survey included the following:

Serum protein levels, as has been noted before, were

generally on the high side of normal; the reason

for this is not apparent. Complementfixation studies

for parainfluenza 1, 2, and 3, respiratory syncitial,
psittacosis, and Q fever showed antibodiesto all
groups of viruses except that for Asian influenza,
which probably had notyet seriously involved the

people of the Marshall Islands. The antibodytiters
appeared to be somewhat lower in the exposed
people. Sodium levels in the urine andfood indicated
about the same consumption of NaCl as in Americans. The generally lower incidence of hypertension in the Marshallese mightbe related to the

fact that the former native diet was probably lower

in salt content than the present, more westernized
diet. It will be interesting to see whether the incidence of hypertension will later increase. Repeat
studies of protein-bound todine, total iodine, and butanolextractable 1edine of the sera showedlevels lower
than previously reported, and the previous higher

readings are thoughtto be in error because of contaminated glassware, although some readings were

still somewhathigh. Four cases of glucosuria associated with elevated blood sugar were found in the
unexposed population, which indicated a rather

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